Merge pull request #157 from zivy/addingReagents

Adding reagent validations from Koenig lab, McMaster University.
IBEXImagingCommunity · Jul 8, 2024 · 24f8c9f · 24f8c9f
2 parents 3de9738 + 83cd9fe
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diff --git a/data/image_resources.csv b/data/image_resources.csv
@@ -164,3 +164,22 @@ CD45_PE/Human_Tonsil_FFPE_Hoechst_blue_CD45_PE_green.jpg,"Human tonsil: Hoechst
 CD8_AF647/Human_Tonsil_FFPE_Hoechst_blue_CD8_red.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CD8 (red, BioLegend cat. no. 372906)",e2a8ea56916b2337125ea81c18050771,"['CD8_AF647/0009-0006-9784-2694.md']"
 CTLA4_AF647/Human_Tonsil_FFPE_Hoechst_blue_CTLA4_red.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and CTLA4 (red, Abcam cat. no. ab270127)",0319ef9d4863816c1d28cfa5e3ec8258,"['CTLA4_AF647/0009-0006-9784-2694.md']"
 PD-1_AF555/Human_Tonsil_FFPE_Hoechst_blue_PD1_green.jpg,"Human tonsil: Hoechst (blue, Biotium cat. no. 40046) and PD-1 (green, Abcam cat. no. ab275126)",32a9ea3243d06baf17be375554c5a9d7,"['PD-1_AF555/0009-0006-9784-2694.md']"
+CD23_AF647/CD23_AF647_Grey_Mouse_LN_2_Large.jpg,"Mouse (naive) lymph node: CD23 (grey, catalog number 101611)",3c6684993361eb1aa6a61f6054b2fd2b,['CD23_AF647/0000-0002-8909-5039.md']
+CD23_AF647/CD23_AF647_Grey_Mouse_LN_1_Large.jpg,"Mouse (immunized) lymph node: CD23 (grey, catalog number 101611)",1e9203e92c26bf6b151ad4872c24a7c7,['CD23_AF647/0000-0002-8909-5039.md']
+CD35_BV510/CD35_BV510_Green_DAPI_Blue_Mouse_LN.jpg,"Mouse (immunized) lymph node: CD35 (green, catalog number 740132) and DAPI (blue, catalog number 62248)",284a4ff657463f54944c9bc42c6cebec,['CD35_BV510/0000-0002-1461-0999.md']
+Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg,"Mouse small intestine: alpha smooth muscle actin (grey, catalogue number 53-9760-82)",51a470bd7b549f77a5d8616442527b67,['Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md']
+B220_AF647/B220_AF647_Grey_Mouse_LN.jpg,"Mouse lymph node: B220 (grey, catalog number 103226)",594441a0105afe2c0f2d0f078e1301f2,['B220_AF647/0000-0002-1461-0999.md']
+CD11b_PE/CD11b_PE_Grey_Mouse_LN.jpg,"Mouse lymph node: CD11b (grey, catalog number 101217)",fb45af754793db4a66ae930241c7613d,['CD11b_PE/0000-0002-8909-5039.md']
+CD11c_AF488/CD11c_AF488_Grey_Mouse_LN.jpg,"Mouse lymph node: CD11c (grey, catalog number MCD11c20)",ee6003913fbc784cae4f0881658c1ff3,['CD11c_AF488/0000-0002-1461-0999.md']
+CD200_BV421/CD200_BV421_Grey_Mouse_LN.jpg,"Mouse lymph node: CD200 (grey, catalog number 565547)",354c6e657d05a678e0406158f51b294d,['CD200_BV421/0000-0002-1461-0999.md']
+CD3_AF488/CD3_488_Grey_Mouse_LN.jpg,"Mouse lymph node: CD3 (grey, catalog number 100210)",87f4c2106f8e91275784e64caca088b7,['CD3_AF488/0000-0002-1461-0999.md']
+CD31_AF594/CD31_AF594_Grey_Mouse_LN.jpg,"Mouse lymph node: CD31 (grey, catalog number 102520)",e96c4e0587f845e94e34f8e74d72cdd6,['CD31_AF594/0000-0002-1461-0999.md']
+CD4_BV421/CD4_BV421_Grey_Mouse_LN_large.jpg,"Mouse lymph node: CD4 (grey, catalog number 100449)",f7e2ff75a88dc016e7b436dc8e120641,['CD4_BV421/0000-0002-1461-0999.md']
+CD4_BV510/CD4_BV510_Grey_Mouse_LN.jpg,"Mouse lymph node: CD4 (grey, catalog number 100443)",ffaa812ed72823857aed78d747ba0306,['CD4_BV510/0000-0002-1461-0999.md']
+CD45.1_AF488/CD45.1_AF488_Magenta_CD138_AF647_Yellow_Mouse_LN.jpg,"Mouse (immunized) lymph node: CD45.1 (magenta, catalog number 110718) and CD138 (yellow, catalog number 142526)",40018e55494cb00bb37f8f5f1210c83c,['CD45.1_AF488/0000-0002-8909-5039.md']
+CD45_AF594/CD45_AF594_Grey_Mouse_LN.jpg,"Mouse lymph node: CD45 (grey, catalog number 103144)",e8e0fabc8fcf9a6d0dd6d9543a535a89,['CD45_AF594/0000-0002-1461-0999.md']
+CD64_AF647/CD64_AF647_Grey_Mouse_LN.jpg,"Mouse lymph node: CD64 (grey, catalog number 139322)",0a8e934ad6cc1de5091f8710a9ed41a9,['CD64_AF647/0000-0002-1461-0999.md']
+CD81_PE/CD81_PE_Grey_Mouse_LN.jpg,"Mouse lymph node: CD81 (grey, catalog number 104905)",aa8dfb29dc0bf9db5e1cc5dfbc24741f,['CD81_PE/0000-0002-8909-5039.md']
+IgD_BV510/IgD_BV510_Green_B220_AF647_Blue_Mouse_LN.jpg,"Mouse lymph node: IgD (green, catalog number 405723) and B220 (blue, catalog number 103226)",cb40bddb73e073c692e25d967d92c70f,['IgD_BV510/0000-0002-1461-0999.md']
+Ki-67_AF488/Ki67_AF488_Grey_Mouse_LN.jpg,"Mouse lymph node: Ki67 (grey, catalog number 558616)",5638e7e89b73d31bc55b1dece18752fc,['Ki-67_AF488/0000-0002-1461-0999.md']
+Pax5_AF647/Pax5_AF647_Margenta_CD3_AF488_Cyan_Mouse_LN.jpg,"Mouse lymph node: Pax5 (magenta, catalog number 649704) and CD3 (cyan, catalog number 100210)",74a7a3125d784c1bdcc0f84fad9f96dc,['Pax5_AF647/0000-0002-1461-0999.md']
diff --git a/data/reagent_resources.csv b/data/reagent_resources.csv
diff --git a/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md b/docs/supporting_material/Alpha_smooth_muscle_actin_AF488/0000-0002-1461-0999.md
@@ -0,0 +1,22 @@
+---
+layout: default
+---
+
+# Configurations
+
+| UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism   | Isotype   | Clonality   | Vendor                   | Catalog Number   | Conjugate   | RRID       | Availability   | Method        | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent         | Antigen Retrieval Conditions   | Dye Inactivation Conditions   | Recommend   | Agree                                                        | Disagree   | Contributor                                                  | Notes   |
+|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
+| P62737                     | Primary Antibody | Alpha smooth muscle actin         | Mouse            | Mouse           | IgG2a     | 1A4         | Thermo Fisher Scientific | 53-9760-82       | AF488       | AB_2574461 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Small Intestine      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes      | Yes         | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA         | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) |         |
+
+# Publications
+
+
+
+# Additional Notes
+
+<a name="notes"></a>
+
+
+| Mouse small intestine: alpha smooth muscle actin (grey, catalogue number 53-9760-82) |
+|:-------:|
+| ![](aSMA_AF488_Grey_Mouse_SI.jpg) |
diff --git a/...upporting_material/Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg b/...upporting_material/Alpha_smooth_muscle_actin_AF488/aSMA_AF488_Grey_Mouse_SI.jpg
diff --git a/docs/supporting_material/B220_AF647/0000-0002-1461-0999.md b/docs/supporting_material/B220_AF647/0000-0002-1461-0999.md
@@ -0,0 +1,22 @@
+---
+layout: default
+---
+
+# Configurations
+
+| UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism   | Isotype   | Clonality   | Vendor    |   Catalog Number | Conjugate   | RRID      | Availability   | Method        | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent         | Antigen Retrieval Conditions   | Dye Inactivation Conditions   | Recommend   | Agree                                                        | Disagree   | Contributor                                                  | Notes   |
+|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
+| NA                         | Primary Antibody | B220                              | Mouse            | Rat             | IgG2a     | RA3-6B2     | Biolegend |           103226 | AF647       | AB_389330 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes      | Yes         | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA         | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) |         |
+
+# Publications
+
+
+
+# Additional Notes
+
+<a name="notes"></a>
+
+
+| Mouse lymph node: B220 (grey, catalog number 103226) |
+|:-------:|
+| ![](B220_AF647_Grey_Mouse_LN.jpg) |
diff --git a/docs/supporting_material/B220_AF647/B220_AF647_Grey_Mouse_LN.jpg b/docs/supporting_material/B220_AF647/B220_AF647_Grey_Mouse_LN.jpg
diff --git a/docs/supporting_material/CD11b_PE/0000-0002-8909-5039.md b/docs/supporting_material/CD11b_PE/0000-0002-8909-5039.md
@@ -0,0 +1,22 @@
+---
+layout: default
+---
+
+# Configurations
+
+| UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism   | Isotype   | Clonality   | Vendor         |   Catalog Number | Conjugate   | RRID      | Availability   | Method        | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent         | Antigen Retrieval Conditions   | Dye Inactivation Conditions   | Recommend   | Agree                                                        | Disagree   | Contributor                                                  | Notes   |
+|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
+| P24788                     | Primary Antibody | CD11b                             | Mouse            | Rat             | IgG2b     | M1/70       | BD Biosciences |           101217 | PE          | AB_396680 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes      | Yes         | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) | NA         | [0000-0002-8909-5039](https://orcid.org/0000-0002-8909-5039) |         |
+
+# Publications
+
+
+
+# Additional Notes
+
+<a name="notes"></a>
+
+
+| Mouse lymph node: CD11b (grey, catalog number 101217) |
+|:-------:|
+| ![](CD11b_PE_Grey_Mouse_LN.jpg) |
diff --git a/docs/supporting_material/CD11b_PE/CD11b_PE_Grey_Mouse_LN.jpg b/docs/supporting_material/CD11b_PE/CD11b_PE_Grey_Mouse_LN.jpg
diff --git a/docs/supporting_material/CD11c_AF488/0000-0002-1461-0999.md b/docs/supporting_material/CD11c_AF488/0000-0002-1461-0999.md
@@ -0,0 +1,22 @@
+---
+layout: default
+---
+
+# Configurations
+
+| UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism    | Isotype   | Clonality   | Vendor                   | Catalog Number   | Conjugate   | RRID        | Availability   | Method        | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent         | Antigen Retrieval Conditions   | Dye Inactivation Conditions   | Recommend   | Agree                                                        | Disagree   | Contributor                                                  | Notes   |
+|:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
+| Q9QXH4                     | Primary Antibody | CD11c                             | Mouse            | Armenian Hamster | IgG       | N418        | Thermo Fisher Scientific | MCD11c20         | AF488       | AB_10373244 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes      | Yes         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) |         |
+
+# Publications
+
+
+
+# Additional Notes
+
+<a name="notes"></a>
+
+
+| Mouse lymph node: CD11c (grey, catalog number MCD11c20) |
+|:-------:|
+| ![](CD11c_AF488_Grey_Mouse_LN.jpg) |
diff --git a/docs/supporting_material/CD11c_AF488/0000-0003-4379-8967.md b/docs/supporting_material/CD11c_AF488/0000-0003-4379-8967.md
@@ -6,7 +6,7 @@ layout: default
 
 | UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism    | Isotype   | Clonality   | Vendor                   | Catalog Number   | Conjugate   | RRID        | Availability   | Method        | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent         | Antigen Retrieval Conditions   | Dye Inactivation Conditions   | Recommend   | Agree                                    | Disagree   | Contributor         | Notes       |
 |:---------------------------|:-----------------|:----------------------------------|:-----------------|:-----------------|:----------|:------------|:-------------------------|:-----------------|:------------|:------------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------|
-| Q9QXH4                     | Primary Antibody | CD11c                             | Mouse            | Armenian Hamster | IgG       | N418        | Thermo Fisher Scientific | MCD11c20         | AF488       | AB_10373244 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes      | Yes         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |
+| Q9QXH4                     | Primary Antibody | CD11c                             | Mouse            | Armenian Hamster | IgG       | N418        | Thermo Fisher Scientific | MCD11c20         | AF488       | AB_10373244 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes      | Yes         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |
 
 # Publications
 

diff --git a/docs/supporting_material/CD11c_AF488/CD11c_AF488_Grey_Mouse_LN.jpg b/docs/supporting_material/CD11c_AF488/CD11c_AF488_Grey_Mouse_LN.jpg
diff --git a/docs/supporting_material/CD200_BV421/0000-0002-1461-0999.md b/docs/supporting_material/CD200_BV421/0000-0002-1461-0999.md
@@ -0,0 +1,22 @@
+---
+layout: default
+---
+
+# Configurations
+
+| UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism   | Isotype   | Clonality   | Vendor         |   Catalog Number | Conjugate   | RRID       | Availability   | Method        | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent         | Antigen Retrieval Conditions   | Dye Inactivation Conditions      | Recommend   | Agree                                                        | Disagree   | Contributor                                                  | Notes   |
+|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
+| Q9ES57                     | Primary Antibody | CD200                             | Mouse            | Rat             | IgG2a     | OX-90       | BD Biosciences |           565547 | BV421       | AB_2739289 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes + light | Yes         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967); [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) |         |
+
+# Publications
+
+
+
+# Additional Notes
+
+<a name="notes"></a>
+
+
+| Mouse lymph node: CD200 (grey, catalog number 565547) |
+|:-------:|
+| ![](CD200_BV421_Grey_Mouse_LN.jpg) |
diff --git a/docs/supporting_material/CD200_BV421/0000-0003-4379-8967.md b/docs/supporting_material/CD200_BV421/0000-0003-4379-8967.md
@@ -6,7 +6,7 @@ layout: default
 
 | UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism   | Isotype   | Clonality   | Vendor         |   Catalog Number | Conjugate   | RRID       | Availability   | Method        | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent         | Antigen Retrieval Conditions   | Dye Inactivation Conditions      | Recommend   | Agree                                    | Disagree   | Contributor         | Notes       |
 |:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:---------------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-----------------------------------------|:-----------|:--------------------|:------------|
-| Q9ES57                     | Primary Antibody | CD200                             | Mouse            | Rat             | IgG2a     | OX-90       | BD Biosciences |           565547 | BV421       | AB_2739289 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes + light | Yes         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)] | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |
+| Q9ES57                     | Primary Antibody | CD200                             | Mouse            | Rat             | IgG2a     | OX-90       | BD Biosciences |           565547 | BV421       | AB_2739289 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node      | NA             | 0.3% Triton-X-100 | NA                             | 1 mg/ml LiBH4 15 minutes + light | Yes         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) [[1](#publications)]; [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | NA         | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |
 
 # Publications
 

diff --git a/docs/supporting_material/CD200_BV421/CD200_BV421_Grey_Mouse_LN.jpg b/docs/supporting_material/CD200_BV421/CD200_BV421_Grey_Mouse_LN.jpg