Merge pull request #201 from SavenDenha/Saven_Denha

new entry for E-cadherin on human tissue

.github/data_validation_configs/reagent_resources.json

@@ -204,7 +204,8 @@
           "Thymus",
           "Tonsil",
           "Tumor",
-          "Uterus"
+          "Uterus",
+          "Nasal Polyp"
        ],
        "Tissue State":[
           "Cancer",

.zenodo.json

@@ -63,6 +63,11 @@
             "name": "Croteau, Joshua",
             "orcid": "0000-0002-8142-0482"
         },
+        {
+            "affiliation": "McMaster University",
+            "name": "Denha, Saven",
+            "orcid": "0009-0004-8162-409X"
+        },
         {
             "affiliation": "National Institutes of Health",
             "name": "Dever, Rose",

data/image_resources.csv

@@ -210,3 +210,5 @@ CXCR3_AF700/Human_tonsil_FFPE_CXCR3_561320.jpg,"Human tonsil FFPE: CXCR3 (cyan,
 CD4_AF488/Human_tonsil_FFPE_CD4_AF488_FAB8165G.jpg,"Human tonsil FFPE: CD4 (green, catalog number FAB8165G)",37fcddd186b1279f72570872be797e13,['CD4_AF488/0000-0002-6604-2065.md']
 CXCR3_AF700/Human_Tonsil_FFPE_CXCR3_FAB160N.jpg,"Human tonsil FFPE: CXCR3 (cyan, catalog number FAB160N)",282723d37e8b3bfc2fe3a57fa805f7bc,['CXCR3_AF700/0000-0002-6604-2065.md']
 CD20_eF615/Human_Tonsil_FFPE_CD20_eF615_42-0202-82.jpg,"Human tonsil FFPE: CD20 (red, catalog number 42-0202-82)",77e6ac7e3a9009daa030e9f17c118f0c,['CD20_eF615/0000-0002-6604-2065.md']
+E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan_EpCAM_Blue.jpg,"Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), EpCAM (blue, catalog number 324228) and DAPI (cyan)",9b79c06f612c26ba7af3cb03c4e21298,['E-cadherin_AF647/0009-0004-8162-409X.md']
+E-cadherin_AF647/Ecadherin_Yellow_DAPI_Cyan.jpg,"Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), and DAPI (cyan)",4dc47ddf0a00a3ad872b91ba448f914a,['E-cadherin_AF647/0009-0004-8162-409X.md']

data/reagent_resources.csv

@@ -1181,4 +1181,5 @@ P01903,Primary Antibody,HLA-DR,Human,Mouse,IgG2a,L243,BioLegend,307622,AF647,AB_
 P49682,Primary Antibody,CXCR3,Human,Mouse,IgG1,1C6/CXCR3,BD Biosciences,561320,AF700,AB_10611719,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,No,0000-0002-6604-2065,NA,0000-0002-6604-2065
 P01730,Primary Antibody,CD4,Human,Goat,IgG,Polyclonal,R&D Systems,FAB8165G,AF488,AB_2728839,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,Yes,0000-0002-6604-2065,NA,0000-0002-6604-2065
 P49682,Primary Antibody,CXCR3,Human,Mouse,IgG1,49801,R&D Systems,FAB160N,AF700,NA,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,No,0000-0002-6604-2065,NA,0000-0002-6604-2065
-P11836,Primary Antibody,CD20,Human,Mouse,IgG2a,L26,Thermo Fisher Scientific,42-0202-82,eF615,AB_10853517,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,Yes,0000-0002-6604-2065,NA,0000-0002-6604-2065
+P11836,Primary Antibody,CD20,Human,Mouse,IgG2a,L26,Thermo Fisher Scientific,42-0202-82,eF615,AB_10853517,Stock,Multiplexed 2D Imaging,FFPE,Tonsil,NA,0.3% Triton-X-100,pH 6 (Sodium Citrate buffer) for 45 minutes,NA,Yes,0000-0002-6604-2065,NA,0000-0002-6604-2065
+P12830,Primary Antibody,E-cadherin,Human,Rat,IgG1,DECMA-1,BioLegend,147307,AF647,AB_2563954,Stock,IBEX2D Manual,1:4 Cytofix/Cytoperm Fixed Frozen,Nasal Polyp,NA,0.3% Triton-X-100,NA,1 mg/ml LiBH4 15 minutes,Yes,0009-0004-8162-409X,NA,0009-0004-8162-409X

docs/supporting_material/E-cadherin_AF647/0009-0004-8162-409X.md

@@ -0,0 +1,23 @@
+---
+layout: default
+---
+
+# Configurations
+
+| UniProt Accession Number   | Reagent Type     | Target Name / Protein Biomarker   | Target Species   | Host Organism   | Isotype   | Clonality   | Vendor    |   Catalog Number | Conjugate   | RRID       | Availability   | Method        | Tissue Preservation               | Target Tissue   | Tissue State   | Detergent   | Antigen Retrieval Conditions   | Dye Inactivation Conditions   | Recommend   | Agree               | Disagree   | Contributor         | Notes       |
+|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------|:-------------------------------|:------------------------------|:------------|:-------------------------------------------------------------|:-----------|:--------------------|:------------|
+| P12830                     | Primary Antibody | E-cadherin                        | Human            | Rat             | IgG1      | DECMA-1     | BioLegend |           147307 | AF647       | AB_2563954 | Stock          | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Nasal Polyp           | NA             | 0.3% Triton-X-100          | NA                             | 1 mg/ml LiBH4 15 minutes      | Yes         | [0009-0004-8162-409X](https://orcid.org/0009-0004-8162-409X)         | NA         | [0009-0004-8162-409X](https://orcid.org/0009-0004-8162-409X) | [1](#notes) |
+
+# Publications
+
+
+# Additional Notes
+
+<a name="notes"></a>
+| Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), and DAPI (cyan) |
+|:-------:|
+| ![](Ecadherin_Yellow_DAPI_Cyan.jpg) |
+
+| Human Nasal Polyp: E-Cadherin (yellow, catalog number 147307), EpCAM (blue, catalog number 324228) and DAPI (cyan) |
+|:-------:|
+| ![](Ecadherin_Yellow_DAPI_Cyan_EpCAM_Blue.jpg) |