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Adding explanation to supporting material. #175

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Jul 8, 2024
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Adding explanation to supporting material. #175

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4 changes: 3 additions & 1 deletion docs/supporting_material/CD4_BV510/0000-0002-1461-0999.md
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Expand Up @@ -6,7 +6,7 @@ layout: default

| UniProt Accession Number | Reagent Type | Target Name / Protein Biomarker | Target Species | Host Organism | Isotype | Clonality | Vendor | Catalog Number | Conjugate | RRID | Availability | Method | Tissue Preservation | Target Tissue | Tissue State | Detergent | Antigen Retrieval Conditions | Dye Inactivation Conditions | Recommend | Agree | Disagree | Contributor | Notes |
|:---------------------------|:-----------------|:----------------------------------|:-----------------|:----------------|:----------|:------------|:----------|-----------------:|:------------|:-----------|:---------------|:--------------|:----------------------------------|:----------------|:---------------|:------------------|:-------------------------------|:---------------------------------|:------------|:-------------------------------------------------------------|:-----------|:-------------------------------------------------------------|:--------|
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | |
| P06332 | Primary Antibody | CD4 | Mouse | Rat | IgG2b | GK1.5 | BioLegend | 100449 | BV510 | AB_2564587 | Stock | IBEX2D Manual | 1:4 Cytofix/Cytoperm Fixed Frozen | Lymph Node | NA | 0.3% Triton-X-100 | NA | 1 mg/ml LiBH4 15 minutes + light | Yes | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [0000-0002-1461-0999](https://orcid.org/0000-0002-1461-0999) | [0000-0003-4379-8967](https://orcid.org/0000-0003-4379-8967) | [1](#notes) |

# Publications

Expand All @@ -20,3 +20,5 @@ layout: default
| Mouse lymph node: CD4 (grey, catalog number 100443) |
|:-------:|
| ![](CD4_BV510_Grey_Mouse_LN.jpg) |

1. The large cells with cytosolic signal in the bottom left of the image do not have the morphology of a CD4+ T cell. This is likely autofluorescence, and it seems to interfere with many dimmer stains on BV510.