SPATAC-seq: split-pool ligation-based scATAC-seq method

Overview of the molecular structure of SPATAC-seq

Dependiencies

• Python 3.0

• BWA

• FASTX-Toolkit

• Cell Ranger ATAC v1.2

• Sinto

• Signac

• ArchR

Citation

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3. Rosenberg AB, Roco CM, Muscat RA, et al. Single-cell profiling of the developing mouse brain and spinal cord with split-pool barcoding. Science. 2018;360(6385):176-182. doi:10.1126/science.aam8999.
4. Ma S, Zhang B, LaFave LM, et al. Chromatin Potential Identified by Shared Single-Cell Profiling of RNA and Chromatin. Cell. 2020;183(4):1103-1116.e20. doi:10.1016/j.cell.2020.09.056.