v0.5.3
Full Changelog: v0.5.0...v0.5.3
primers score SEQ1 SEQ2 -s TEMPLATE_SEQ
now returns separatetm
andtm_total
s. This affects score:
$ primers score GGTCTCAATGAGACAATA AAAAAATTTCGTATGCTGACCTAG -s AATGAGACAATAGCACACACAGCTAGGTCAGCATACGAAA --json | jq
[
{
"seq": "GGTCTCAATGAGACAATA",
"len": 18,
"tm": 39.4,
"tm_total": 55,
"gc": 0.4,
"dg": -1.86,
"fwd": true,
"off_target_count": 0,
"scoring": {
"penalty": 49.9,
"penalty_tm": 22.6,
"penalty_tm_diff": 19.6,
"penalty_gc": 2,
"penalty_len": 2,
"penalty_dg": 3.7,
"penalty_off_target": 0
}
},
{
"seq": "AAAAAATTTCGTATGCTGACCTAG",
"len": 24,
"tm": 59,
"tm_total": 61.6,
"gc": 0.4,
"dg": 0,
"fwd": false,
"off_target_count": 0,
"scoring": {
"penalty": 25.6,
"penalty_tm": 3,
"penalty_tm_diff": 19.6,
"penalty_gc": 2,
"penalty_len": 1,
"penalty_dg": 0,
"penalty_off_target": 0
}
}
]