Track nuclei in a time series of 2D images. The images are first filtered by Gaussian blur (user defined radius), thresholded (user defined level) and binary watershed is applied. Next, erosion is performed (user defined radius) and the particles belonging to the same 3D tube (mask series viewed as 3D mask) are labeled with same ID. The particles are found by ultimate point erosion of the 2D masks.
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Neubias-WG5/W_NucleiTracking-ImageJ
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BIAFLOWS workflow to track nuclei in a time series. The tracking uses a 3D-object segmentation with watershed to track the nuclei.
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