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Input files: Go-terms of interest: From the GO_annotations.xlsx (or Slim_Go.xlsx - in Project_Datasets), copy the go terms you are interested in into Go_terms_OI.xlsx and replace the defaults. Proteins of interest: In POI.xlsx replace the default protein names with your own proteins that you want to rank (It accepts any type of protein name, e.g. AACS,SPCS_HUMAN,P21796 are all recognised Should be added as in the examples in the Dataset Folder code is: POI_list <- read.xlsx("./../Project_Datasets/POIS.xlsx", colNames = F) Go_terms_OI <- read.xlsx("./../Project_Datasets/Go_terms_OI.xlsx", colNames = F)$X1 EnrichGO can only occur for gene lists >10 so some are excluded EnrichmentGo clusterProfiler Explanation from https://www.biostars.org/p/220465/ I will give an example to explain this that helped me understand it. I also was looking for the answer and Guangchuang link helped. Let is suppose I have a collection of genesets called : HALLMARK Now let is suppose there is a specific geneset there called: E2F_targets BgRatio, M/N. M = size of the geneset (eg size of the E2F_targets); (is the number of genes within that distribution that are annotated (either directly or indirectly) to the node of interest). N = size of all of the unique genes in the collection of genesets (example the HALLMARK collection); (is the total number of genes in the background distribution (universe) GeneRatio is k/n. k = size of the overlap of 'a vector of gene id' you input with the specific geneset (eg E2F_targets), only unique genes; (the number of genes within that list n, which are annotated to the node. n = size of the overlap of 'a vector of gene id' you input with all the members of the collection of genesets (eg the HALLMARK collection),only unique genes; is the size of the list of genes of interest
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ConsensusDB Overrepresenation analysis based on partners
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