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AdvancedImagingUTSW authored Oct 25, 2024
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61 changes: 61 additions & 0 deletions docs/source/user_guide/communication_challenges.rst
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========================
Hardware Troubleshooting
========================

In this section, we outline some common challenges encountered in setting up a
microscope system and provide solutions to address these issues effectively.

-------------------------------------

.. _obis_tiger_connection:

Intermittent USB Connectivity
#############################

Overview
--------
For setups that include **Coherent OBIS lasers** and an **ASI Tiger Controller**, some
users have reported intermittent connection issues. These errors
were first observed at CU Boulder, where the Coherent OBIS laser and the ASI
Tiger controller appeared to experience conflicts over COM port assignments.

Reported Issues
---------------
1. **Connection Failure with the ASI Stage**:
- The ASI stage intermittently fails to connect, with an error message reading:
"Unable to connect to the serial port: Access to the port ‘COM13’ is denied."
- This error has occurred in both the Tiger control software and *navigate*.

2. **Error Logs in Navigate**:
- In the navigate software, the error is logged as:
`"model - ERROR - asi: ASI stage connection failed"`.

3. **Stage Malfunction During Imaging**:
- When a connection was achieved, the ASI stages exhibited sporadic movements during
image acquisition.

Suspected Cause
---------------
Upon investigation, the issue appeared to stem from **port competition** between the Coherent OBIS laser and the ASI Tiger controller. In this setup:
- The ASI Tiger controller was assigned to **COM port 13**.
- The Coherent OBIS 561 nm laser was initially assigned to **COM port 14**.

Despite this configuration, the Coherent OBIS laser intermittently attempted to connect through COM port 13, effectively displacing the ASI Tiger controller's access to the port and causing a conflict.

Solution and Verification
-------------------------
To address this issue, the following corrective actions were implemented:

1. **Reassigning the Coherent OBIS Laser**:
- The Coherent OBIS laser was reassigned from COM port 14 to **COM port 9** to
prevent interference with the ASI Tiger controller. This port was chosen since it was
in close proximity to the other Coherent OBIS laser instances.

2. **System Reboot**:
- Following the reassignment, both the computer and hardware were rebooted to ensure the new COM port configurations took effect.

Results
-------
Since reassigning the Coherent OBIS laser to COM port 9, the ASI stage has maintained
a stable connection on COM port 13 without interruptions and uncontrolled stage
movements are no longer occurring.
7 changes: 7 additions & 0 deletions docs/source/user_guide/hardware/laser.rst
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Expand Up @@ -25,6 +25,13 @@ modulation.
for the slew rate from the data acquisition card to be insufficient to drive the modulation
of the laser if the laser is set to an analog modulation mode.

.. note::
Users have reported intermittent connection issues at random intervals arising
from USB-based communication instance with Coherent Obis controllers. Specifically,
these errors were observed as a conflict over COM port assignments between the
Coherent OBIS laser and the ASI Tiger controller. These issues are discussed in
depth in the :ref:`communication challenges <obis_tiger_connection>` section.


.. collapse:: Configuration File

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5 changes: 4 additions & 1 deletion docs/source/user_guide/hardware/stage.rst
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Expand Up @@ -40,7 +40,10 @@ first establish communication with the device using `ASI provided software <http

- Tiger Controller 2.2.0.


.. note::
Some users have reported intermittent connection issues at random intervals when
used with Coherent OBIS lasers. These issues, and how to address them, are discussed
in the :ref:`communication challenges <obis_tiger_connection>` section.

.. warning::
If you are using the FTP-2000 stage, do not change the F stage axis. This
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1 change: 1 addition & 0 deletions docs/source/user_guide/setup_microscope.rst
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software_configuration
multiple_cameras
communication_challenges

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