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======================== | ||
Hardware Troubleshooting | ||
======================== | ||
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In this section, we outline some common challenges encountered in setting up a | ||
microscope system and provide solutions to address these issues effectively. | ||
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------------------------------------- | ||
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.. _obis_tiger_connection: | ||
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Intermittent USB Connectivity | ||
############################# | ||
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Overview | ||
-------- | ||
For setups that include **Coherent OBIS lasers** and an **ASI Tiger Controller**, some | ||
users have reported intermittent connection issues. These errors | ||
were first observed at CU Boulder, where the Coherent OBIS laser and the ASI | ||
Tiger controller appeared to experience conflicts over COM port assignments. | ||
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Reported Issues | ||
--------------- | ||
1. **Connection Failure with the ASI Stage**: | ||
- The ASI stage intermittently fails to connect, with an error message reading: | ||
"Unable to connect to the serial port: Access to the port ‘COM13’ is denied." | ||
- This error has occurred in both the Tiger control software and *navigate*. | ||
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2. **Error Logs in Navigate**: | ||
- In the navigate software, the error is logged as: | ||
`"model - ERROR - asi: ASI stage connection failed"`. | ||
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3. **Stage Malfunction During Imaging**: | ||
- When a connection was achieved, the ASI stages exhibited sporadic movements during | ||
image acquisition. | ||
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Suspected Cause | ||
--------------- | ||
Upon investigation, the issue appeared to stem from **port competition** between the Coherent OBIS laser and the ASI Tiger controller. In this setup: | ||
- The ASI Tiger controller was assigned to **COM port 13**. | ||
- The Coherent OBIS 561 nm laser was initially assigned to **COM port 14**. | ||
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Despite this configuration, the Coherent OBIS laser intermittently attempted to connect through COM port 13, effectively displacing the ASI Tiger controller's access to the port and causing a conflict. | ||
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Solution and Verification | ||
------------------------- | ||
To address this issue, the following corrective actions were implemented: | ||
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1. **Reassigning the Coherent OBIS Laser**: | ||
- The Coherent OBIS laser was reassigned from COM port 14 to **COM port 9** to | ||
prevent interference with the ASI Tiger controller. This port was chosen since it was | ||
in close proximity to the other Coherent OBIS laser instances. | ||
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2. **System Reboot**: | ||
- Following the reassignment, both the computer and hardware were rebooted to ensure the new COM port configurations took effect. | ||
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Results | ||
------- | ||
Since reassigning the Coherent OBIS laser to COM port 9, the ASI stage has maintained | ||
a stable connection on COM port 13 without interruptions and uncontrolled stage | ||
movements are no longer occurring. |
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software_configuration | ||
multiple_cameras | ||
communication_challenges |