Sequencing-based research has become the dominant investigative practice within the biological sciences. This repository, "Single-molecule sequencing: training, methods, and applications," provides a comprehensive, hands-on, and in-depth introduction to sequencing technologies for students. Covering techniques from Sanger to third-generation technologies, this course alternates between lectures and labs, introducing students to the fundamental principles of DNA and RNA science. It progresses through cutting-edge library preparation and sequencing analysis techniques using Illumina and Nanopore technologies. Participants will perform direct-RNA sequencing on research samples and explore the ethical implications of data handling. The course also integrates relevant concepts in biology and computer science.
This repository is organized into course modules, each contained in separate folders corresponding to the course structure. Below is a description of each folder and its contents.
- L1_Single-Molecule-Sequencing_Methods_Training_Applications_V3.pdf: Slide deck covering the course introduction & motivation, single-molecule fluorescence, and traditional next-generation sequencing technologies.
- HW1_Notebook_Setup_Single_Molecule_Sequencing_Methods_Training_Applications.ipynb: Google Colab setup notebook.
To get started with the course materials in this repository, clone the repository to your local machine using the following command:
git clone [https://github.com/Theo-Nelson/SMS-colab.git](https://github.com/Theo-Nelson/SMS-colab.git)
git