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tives82 committed Mar 5, 2024
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Expand Up @@ -49,6 +49,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool
> **Clean read data undergo assembly and influenza typing and subtyping. Based on the subtype information, Nextclade variables are gathered.**
* Assembly of influenza gene segments with (`IRMA`) using the built-in FLU module. Also, influenza typing and H/N subtype classifications are made.
* Calculate the reference length, sequence length, and percent_coverage for segments assembled by IRMA with (`IRMA_SEGMENT_COVERAGE`)
* Calculate the number of mapped reads and mean depth for segments assembled by IRMA with (`SAMTOOLS_MAPPED_READS`)
* QC of consensus assembly (`IRMA_Consensus_QC`).
* Generate IRMA consensus QC report (`IRMA_Consensus_QC_Reportsheet`)
* Annotation of IRMA consensus sequences with (`VADR`)
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> **Compiles report sheets from modules and outputs a pipeline summary report tsv file.**
* The (`Summary_Report`) consolidates and merges multiple report sheets into a single comprehensive summary report.
* The (`merged_bam_coverage_results`) merges the gene segment report sheets detailing mapped reads, mean depth, reference length, sequence length, and percent_coverage.

## Quick Start

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