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add crop instruction
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veegalinova committed Feb 8, 2024
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6 changes: 3 additions & 3 deletions README.md
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Expand Up @@ -115,8 +115,8 @@ Crop the empty background:
<div>
<img src="resources/tutorial_images/fiji_scale2.png" width="30%">
</div>
2. Press `Edit › Selection › Select Bounding Box` to select the non-empty region
3. Press `Image › Crop` to crop the region
2. Press `Edit › Selection › Select Bounding Box` to select the non-empty region. If it did not work, try using `Select Bounding Box (guess background color)`
3. Press `Image › Crop` to crop the region.
4. You can scale the image to reduce its size for faster pipeline performance.
Press `Image > Scale...` and enter the desired scale, like 0.5 or 0.25.

Expand Down Expand Up @@ -162,7 +162,7 @@ To link cells between images we will be using a plugin designed for tracking - [
3. Open up TrackMate `Plugins > Tracking > TrackMate`
4. In the first window, it's possible select out the region you want to process and click `Refresh ROI `
5. In the next step, select the `Cellpose Detector`
6. Check if `Path to cellpose` is correct. It should be the same location that you installed your cellpose into. It should look like this: `/Users/emilianagiacomello/miniforge3/envs/cellpose/bin/python`
6. Check if `Path to cellpose` is correct. It should be the same location that you installed your cellpose into. It should look like: `/Users/emilianagiacomello/miniforge3/envs/cellpose/bin/python`
7. Select `Custom` model from `Pretrained model` and add a path to the downloaded model.
8. Check if the approximate diameter of your objects in `Cell diameter` is correct. Here is an example of final settings:
<div>
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