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At this moment here are three files: 1) valid_gtf.pl 2) mRNA2genomeBAM.pl 3) example.sh 4) take_fasta_from_gtf.pl 5) wig_parse.pl 6) correct_gtf_chains.pl valid_gtf.pl validation of gtf is neeeded when our transcript names differin BAM and gtf. At now 'valid_gtf.pl' takes standard GoldenPath RefGene.txt and creates file for Bowtie. You have to keep in mind that fact that output of 'main_current.pl' now is in SAM format, so after it's job have be done, you need to create BAM, sort it and then make index file. for samtools, it will be as: $ samtools view -bS output.sam > output.bam $ samtools sort output.bam output_sorted $ samtools index output_sorted.bam mRNA2genomeBAM.pl Usage of mRNA2genomeBAM.pl is described in it's help. Try '-h' or '--help'. TAKE_FASTA_FROM_GTF.PL This script makes fasta RefSeq file from validated GTF files and genome reference WIG_PARSE.PL This script parses WIG file and create GTF table that contains raws with continious regions. Actually, it's a very simple peakfinder correct_gtf_chains.pl Made for poor peakfinders that don't track directions of reads in peaks. some other scripts NOTES DEPENDENCIES samtools libbio-perl-samtools perl :) TODO 1. make correct_gtf_chains.pl to work 2. Some tetriary analisys scripts for validate new undescribed transcripts and miRNAs 3. statistical analisys for differential expression levels for peaks and transcripts
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