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added option to read in masked positions as a file #181

added option to read in masked positions as a file

added option to read in masked positions as a file #181

Workflow file for this run

name: "polyphonia CI"
on:
push:
pull_request:
branches:
- master
release:
types:
- created
schedule:
- cron: '0 18 * * 1' # weekly at 18:00 on Mondays
env:
HOME: "${{ github.workspace }}"
CACHE_DIR: "${{ github.workspace }}/misc_cache"
MINICONDA_DIR: "${{ github.workspace }}/miniconda"
PYTHONIOENCODING: UTF8
GITHUB_ACTIONS_COMMIT: ${{ github.sha }}
GITHUB_ACTIONS_BUILD_DIR: ${{ github.workspace }}
GITHUB_ACTIONS_BRANCH: ${{ github.ref }}
GITHUB_ACTIONS_PULL_REQUEST: ${{ github.event.number }}
GITHUB_ACTIONS_PULL_REQUEST_BRANCH: ${{ github.head_ref }}
GITHUB_ACTIONS_PULL_REQUEST_SHA : ${{ github.event.pull_request.head.sha }}
GITHUB_ACTIONS_BASE_REF: ${{ github.base_ref }}
DOCKER_REGISTRY: "quay.io"
DOCKER_REPO_PROD: "quay.io/broadinstitute/polyphonia"
DOCKER_REPO_DEV: "quay.io/broadinstitute/polyphonia"
DOCKER_POLYPHONIA_PATH: "/opt/polyphonia"
jobs:
test_build_docker:
runs-on: ubuntu-20.04
steps:
- name: checkout repository
uses: actions/checkout@v2
- name: fetch tags
run: git fetch --prune --unshallow --tags
- name: Programmatic environment setup
run: |
set -e -x
# $GITHUB_ENV is available for subsequent steps
GITHUB_ACTIONS_TAG=$(git describe --tags --exact-match && sed 's/^v//g' || echo '')
echo "GITHUB_ACTIONS_TAG=$GITHUB_ACTIONS_TAG" >> $GITHUB_ENV
#
# Set GITHUB_ACTIONS_BRANCH
# TRAVIS_BRANCH: (via https://docs.travis-ci.com/user/environment-variables/ )
# for push builds, or builds not triggered by a pull request, this is the name of the branch.
# for builds triggered by a pull request this is the name of the branch targeted by the pull request.
# for builds triggered by a tag, this is the same as the name of the tag (TRAVIS_TAG).
GITHUB_ACTIONS_BRANCH=$(git rev-parse --abbrev-ref HEAD)
if [ -n "$GITHUB_ACTIONS_PULL_REQUEST_BRANCH" ]; then
GITHUB_ACTIONS_BRANCH=${GITHUB_ACTIONS_BASE_REF##*/}
elif [[ "${{ github.event_name }}" == "release" ]]; then
GITHUB_ACTIONS_BRANCH=$GITHUB_ACTIONS_TAG
fi
echo "GITHUB_ACTIONS_BRANCH=$GITHUB_ACTIONS_BRANCH" >> $GITHUB_ENV
echo "GITHUB_ACTIONS_SHORT_SHA=$(echo $GITHUB_SHA | head -c8)" >> $GITHUB_ENV
- name: Set up Docker Buildx
uses: docker/setup-buildx-action@v1
- name: Cache Docker layers
uses: actions/cache@v2
with:
path: /tmp/.buildx-cache
key: ${{ runner.os }}-buildx-${{ github.sha }}
restore-keys: |
${{ runner.os }}-buildx-
- name: Build and export
id: docker_build
uses: docker/build-push-action@v2
with:
context: .
push: false
tags: "polyphonia:${{ env.GITHUB_ACTIONS_BRANCH }}-${{ env.GITHUB_ACTIONS_SHORT_SHA }}"
cache-from: type=local,src=/tmp/.buildx-cache
cache-to: type=local,dest=/tmp/.buildx-cache-new
- name: Image digest
run: echo ${{ steps.docker_build.outputs.digest }}
# Temp fix
# https://github.com/docker/build-push-action/issues/252
# https://github.com/moby/buildkit/issues/1896
- name: Store build in cache (overwrite)
run: |
rm -rf /tmp/.buildx-cache
mv /tmp/.buildx-cache-new /tmp/.buildx-cache
test_execution:
runs-on: ubuntu-20.04
needs: test_build_docker
steps:
- name: checkout repository
uses: actions/checkout@v2
- name: fetch tags
run: git fetch --prune --unshallow --tags
- name: Programmatic environment setup
run: |
set -e -x
# $GITHUB_ENV is available for subsequent steps
GITHUB_ACTIONS_TAG=$(git describe --tags --exact-match && sed 's/^v//g' || echo '')
echo "GITHUB_ACTIONS_TAG=$GITHUB_ACTIONS_TAG" >> $GITHUB_ENV
#
# Set GITHUB_ACTIONS_BRANCH
# TRAVIS_BRANCH: (via https://docs.travis-ci.com/user/environment-variables/ )
# for push builds, or builds not triggered by a pull request, this is the name of the branch.
# for builds triggered by a pull request this is the name of the branch targeted by the pull request.
# for builds triggered by a tag, this is the same as the name of the tag (TRAVIS_TAG).
GITHUB_ACTIONS_BRANCH=$(git rev-parse --abbrev-ref HEAD)
if [ -n "$GITHUB_ACTIONS_PULL_REQUEST_BRANCH" ]; then
GITHUB_ACTIONS_BRANCH=${GITHUB_ACTIONS_BASE_REF##*/}
elif [[ "${{ github.event_name }}" == "release" ]]; then
GITHUB_ACTIONS_BRANCH=$GITHUB_ACTIONS_TAG
fi
echo "GITHUB_ACTIONS_BRANCH=$GITHUB_ACTIONS_BRANCH" >> $GITHUB_ENV
echo "GITHUB_ACTIONS_SHORT_SHA=$(echo $GITHUB_SHA | head -c8)" >> $GITHUB_ENV
- name: Set up Docker Buildx
uses: docker/setup-buildx-action@v1
- name: Cache Docker layers
uses: actions/cache@v2
with:
path: /tmp/.buildx-cache
key: ${{ runner.os }}-buildx-${{ github.sha }}
restore-keys: |
${{ runner.os }}-buildx-
# re-build image (from cache; this seems faster than exporting the image and passing as an Actions artifact)
- name: Build and export
id: docker_build
uses: docker/build-push-action@v2
with:
context: .
push: false
load: true
tags: "polyphonia:${{ env.GITHUB_ACTIONS_BRANCH }}-${{ env.GITHUB_ACTIONS_SHORT_SHA }}"
cache-from: type=local,src=/tmp/.buildx-cache
- name: Image digest
run: echo ${{ steps.docker_build.outputs.digest }}
- name: Create local dir for test output
run: |
mkdir -p output
- name: Run polyphonia (basic execution test)
run: |
docker run -v $(pwd)/bin:${{ env.DOCKER_POLYPHONIA_PATH }} -v $(pwd)/test:${{ env.DOCKER_POLYPHONIA_PATH }}/test -v $(pwd)/output:/tmp/output --rm polyphonia:${{ env.GITHUB_ACTIONS_BRANCH }}-${{ env.GITHUB_ACTIONS_SHORT_SHA }} \
polyphonia cross_contamination
- name: Run polyphonia (cross_contamination test)
run: |
set -e -x
docker run -v $(pwd)/bin:${{ env.DOCKER_POLYPHONIA_PATH }} -v $(pwd)/test:${{ env.DOCKER_POLYPHONIA_PATH }}/test -v $(pwd)/output:/tmp/output --rm polyphonia:${{ env.GITHUB_ACTIONS_BRANCH }}-${{ env.GITHUB_ACTIONS_SHORT_SHA }} \
polyphonia cross_contamination \
--ref ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/NC_045512.2.fasta \
--vcf ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315-2021.bam_LoFreq.vcf \
--vcf ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01323-2021.bam_LoFreq.vcf \
--consensus ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315-2021.fasta \
--consensus ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01323-2021.fasta \
--plate-map ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315_23-2021_plate_map.txt \
--min-depth 0 \
--output /tmp/output/potential_cross-contamination.txt \
--overwrite TRUE
DIFF=$(diff $(pwd)/output/potential_cross-contamination.txt $(pwd)/test/output/potential_cross-contamination.txt)
if [ "$DIFF" != "" ]; then
echo "Failure: cross_contamination test output does not match expected output"
echo " expected output:"
cat $(pwd)/output/potential_cross-contamination.txt
echo " test output:"
cat ${{ env.DOCKER_POLYPHONIA_PATH }}/test/output/potential_cross-contamination.txt
exit 1
else
echo "Success: cross_contamination test"
fi
- name: Run polyphonia (cross_contamination test with aligned consensus sequences)
run: |
set -e -x
docker run -v $(pwd)/bin:${{ env.DOCKER_POLYPHONIA_PATH }} -v $(pwd)/test:${{ env.DOCKER_POLYPHONIA_PATH }}/test -v $(pwd)/output:/tmp/output --rm polyphonia:${{ env.GITHUB_ACTIONS_BRANCH }}-${{ env.GITHUB_ACTIONS_SHORT_SHA }} \
polyphonia cross_contamination \
--ref ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/NC_045512.2.fasta \
--vcf ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315-2021.bam_LoFreq.vcf \
--vcf ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01323-2021.bam_LoFreq.vcf \
--consensus-aligned ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315_23-2021_MAFFT_aligned.fasta \
--plate-map ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315_23-2021_plate_map.txt \
--min-depth 0 \
--output /tmp/output/potential_cross-contamination.txt \
--overwrite TRUE
DIFF=$(diff $(pwd)/output/potential_cross-contamination.txt $(pwd)/test/output/potential_cross-contamination.txt)
if [ "$DIFF" != "" ]; then
echo "Failure: cross_contamination test output does not match expected output"
echo " expected output:"
cat $(pwd)/output/potential_cross-contamination.txt
echo " test output:"
cat ${{ env.DOCKER_POLYPHONIA_PATH }}/test/output/potential_cross-contamination.txt
exit 1
else
echo "Success: cross_contamination test"
fi
- name: Run polyphonia (cross_contamination test with aligned consensus sequences with gap in reference)
run: |
set -e -x
docker run -v $(pwd)/bin:${{ env.DOCKER_POLYPHONIA_PATH }} -v $(pwd)/test:${{ env.DOCKER_POLYPHONIA_PATH }}/test -v $(pwd)/output:/tmp/output --rm polyphonia:${{ env.GITHUB_ACTIONS_BRANCH }}-${{ env.GITHUB_ACTIONS_SHORT_SHA }} \
polyphonia cross_contamination \
--ref ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/NC_045512.2.fasta \
--vcf ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315-2021.bam_LoFreq.vcf \
--vcf ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01323-2021.bam_LoFreq.vcf \
--consensus-aligned ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315_23-2021_MAFFT_aligned_gap_added.fasta \
--plate-map ${{ env.DOCKER_POLYPHONIA_PATH }}/test/input/USA-MA-Broad_CRSP-01315_23-2021_plate_map.txt \
--min-depth 0 \
--output /tmp/output/potential_cross-contamination.txt \
--overwrite TRUE
DIFF=$(diff $(pwd)/output/potential_cross-contamination.txt $(pwd)/test/output/potential_cross-contamination.txt)
if [ "$DIFF" != "" ]; then
echo "Failure: cross_contamination test output does not match expected output"
echo " expected output:"
cat $(pwd)/output/potential_cross-contamination.txt
echo " test output:"
cat ${{ env.DOCKER_POLYPHONIA_PATH }}/test/output/potential_cross-contamination.txt
exit 1
else
echo "Success: cross_contamination test"
fi