Media Recipes

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N-C- Base Minimal Medium

Brief Description

N-C- Minimal Medium is effectively a phosphate buffered base medium for growth of heterotrophic microbes. It is typically stored as a 10X stock to which carbon and nitrogen sources are later added to make a complete medium capable of supporting microbial growth. It is a high-osmolarity medium, so care should be taken when adding in large amounts of Carbon and Nitrogen sources.

Composition

Component	Concentration [mM]	Function	Vendor & Catalog Number
$K_2SO_4$	5.7	Sulfate Source	Sigma-Aldrich: P0772-1KG
$K_2HPO_4$	77.6	Buffering + Phosphate Source
$KH_2PO_4$	34.6	Buffering + Phosphate Source
$MgSO_4$	0.4	Magnesium Source
$NaCl$	43.1	Sodium Source
Carbon Source	Variable	Carbon Source	Variable
$NH_4Cl$	10	Nitrogen Source
Micronutrients	Variable	Trace Element Source	See Note Below

It’s recommended that you add micronutrients to ensure reproducibility between culturing as different water sources and different glasswares have different levels of purity.

Recipe

It’s recommended to store the N-C- buffer base as a filter-sterilized 4x stock. This has a long shelf life (> 2 years) and is robust to contamination as it doesn’t have either a nitrogen or carbon source.

500 mL of a 4X N-C- Base Buffer

Component	Quantity	Note
$K_2SO_4$	2 g
$K_2HPO_4$	27 g	Can also use 35.4 g of $K_2HPO_4\cdot 3H_2O$
$KH_2PO_4$	9.4 g
$MgSO_4$	0.8 mL of 1M stock	Can also add 0.2 g of $MgSO_4\cdot 7H_2O$
$NaCl$	5g
dd $H_2O$	to 500 mL

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