This a a package designed for trangenic integration and rearrangement sites discovery using sequencing data
library(devtools)
install_github("menggf/transgeneR")
TransgeneR uses bowtie2 as the alignment tool. Therefore, before usage, bowtie2 must have been installed in the linux mechine and its location has been added to $PATH variable. Meanwhile, the genome reference index has been constructed using "bowtie2-build" command.
test.data=system.file("extdata", "test.zip", package = "transgeneR")
temp <- tempdir()
unzip(test.data, exdir=temp)
output.dir=paste(temp,"/test",sep="")
transgene.sq=paste(temp,"/test/temp_files/insert.fa",sep="")
transgeneR(output.dir, insert.seq=transgene.sq)
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error: /usr/bin/ld: cannot find -lboost_iostreams
Please install boost library in your system using the command like:
yum install boost-devel