Circadian period Q₁₀ calculator

This is a simple code to calculate Q₁₀ values for circadian period length by using SciPy Optimize non-linear least squares fit on Google Colab.

This program is for calculating Q₁₀ values and plotting data only. Your data entered to this calculator will not be recorded or stored in any location (please see the code).

This calculator fits an exponential equation:

Tau_t = Tau₃₇ / ( Q₁₀ ** ( ( t - 37 ) * 0.1 ) )

to a set of data points, and estimates a period length at 37˚C (Tau₃₇) and temperature coefficient (Q₁₀).

For cyanobacteria data, it fits an exponential equation:

Tau_t = Tau₃₀ / ( Q₁₀ ** ( ( t - 30 ) * 0.1 ) )

to a set of data points, and estimates a period length at 30˚C (Tau₃₀) and temperature coefficient (Q₁₀).

Usage

Open the calculator (Google Colab notebook)

Click on the link below:
https://colab.research.google.com/github/dxda6216/q10/blob/main/circadian_period_q10.ipynb

For cyanobacteria data, please use:
https://colab.research.google.com/github/dxda6216/q10/blob/main/circadian_period_q10_for_cyano.ipynb

Note: To use the Colab, you will need to login to your Google account.

Sample Description

To the "Data_description" input form, enter a description of your sample. This field can be blank.

Data_description: Enter a description of your sample. 

Temperature and Period Data

Enter your data into the "Temperatures" and "Periods" input forms. For example, if you have the following dataset

Temp (˚C)   Period (hr)
  32.2        28.5
  35.3        27.1
  37.0        24.2
  39.6        22.6
  41.8        20.7

enter your data as follows:

Temperatures: 32.2, 35.3, 37.0, 39.6, 41.8     
Periods: 28.5, 27.1, 24.2, 22.6, 20.7          

The values should be delimited by a single comma. Leading and trailing spaces will be ignored.

Data Plotting

Scales of X-axis and Y-axis, ticks, etc will automatically be adjusted.

Exporting Dataset and Fitted Curve

To output the dataset and fitted curve in tab-delimited format, select "Yes" in the "Display_tab_delimited_data" dropdown, and 2-column datasets will be displayed. To export the data to Excel, copy the 2-column dataset and paste it onto an Excel worksheet (right click -> Paste Options: Paste Special... -> Paste as Unicode Text -> click "OK").

If you have period data obtained only at 2 different temperatures (only 2 data points), please see: https://github.com/dxda6216/q10_two_temperatures