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Sequencing-and-assembly

  • 拟南芥 (Arabidopsis)
  • 蒺藜苜蓿 (Medicago truncatula)
  • 大豆 (cicer arietinum)
  • 兵豆 (Glycine max)
  • 红豆 (Vigna angularis)
  • 豌豆 (Pisum sativum)
  • 百脉根 (Lotus corniculatus)
  • 鹰嘴豆 (cicer arietinum)
# Anchr的参数说明

basename :the basename of this genome, default is the working directory
         :基因组文件的基名,默认是工作目录
 
genome   :your best guess of the haploid genome size
         :最好知道单倍体基因组的大小

is_euk   :eukaryotes or not
         :是否为真核生物

tmp=s    :user defined tempdir
         :用户定义的临时文件夹

se       :single end mode for Illumina
         :Illumina的单端测序模式

trim2=s  :steps for trimming Illumina reads
         :修剪reads的步骤(默认是--uniq)

sample2=i:total sampling coverage of Illumina reads
         :Illumina reads的所有样品的覆盖度

cov2=s   :down sampling coverage of Illumina reads
         :从测序文件中提取多少量的read进行组装(因为不同深度对于不同细胞器的组装的影响是不同的)(默认是40 80)

qual2=s  :quality threshold
         :read质量控制的阈值【二代】(默认是25 30)

len2=s   :filter reads less or equal to this length
         :将长度短于该值的read筛选掉(默认是60)

reads=s  :how many reads to estimate insert size
         :使用多少条reads来对插入片段进行估计(默认是2000000条)

tadpole  :also use tadpole to create k-unitigs(不太理解)
         :使用tadpole来创建k-unitigs

cov3=s   :down sampling coverage of PacBio reads
         :同cov2【三代】

qual3=s  :raw and/or trim
         :是使用原始数据还是对数据进行修剪【三代】(默认是“修剪”)

parallel :number of threads
         :运行的的线程数

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😗some species plastid genome assemble

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