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Snakebite-RNAseq

Standard RNA-seq snakemake pipeline.

Installation

The test data is not part of this repository and needs to be downloaded separately here:

wget ...

To clone into the pipeline type

git clone git@github.com:fischuu/Snakebite-RNAseq.git

Usage

The pipeline is configured in the file ... with the following required parameters.

In addition, the follwoing must be provided

Preparations:

Create rawsamples files

Create samples files

Create sampleInfo.txt file

cut -d'-' -f1 samples > animal cut -d'-' -f2 samples > pathogen cut -d'-' -f3 samples > time cut -d'-' -f4 samples > replicate cut -d'-' -f5 samples > sequencer

paste -d'\t' samples animal pathogen time replicate sequencer > sampleList.tmp

rm animal rm time rm sequencer rm pathogen rm replicate

printf "sample\tanimal\tpathogen\ttime\treplicate\tsequencer\n" > header cat header sampleList.tmp > sampleInfo.txt rm sampleList.tmp rm header

Current status

Under development and possible not yet stable for production