...running with pure JavaScript in your browser - no need to install anything.
The Wily-DNA-Editor at wily-dna-editor.com
The Wily-DNA-Editor at www.gear-genomics.com/wily-dna-editor
Source code is available at https://github.com/gear-genomics/wily-dna-editor
git clone https://github.com/gear-genomics/wily-dna-editor.git
Reverse-Complement DNA, remove non-IUPAC letters and change upper/lower case...
Find restriction sites, select them and highlight the selected in sequence...
Draw restriction maps with or without features for linear an circular sequences...
Simulate band indensity or draw all bands black...
Translate DNA in one, three or six frames...
Use features to annotate regions in the DNA...
All calculation happens on your machine. You can load and save sequences in fasta and genbank format. Pictures can be saved as html or svg files to disk and modified for publication...