Introduction to RNA-seq using Salmon Lessons from HCBC (first release)

Description

This workshop focuses on teaching basic computational skills to enable the effective use of an high-performance computing environment to implement an RNA-seq data analysis workflow. It includes an introduction to shell (bash) and shell scripting. In addition to running the RNA-seq workflow from FASTQ files to count data using Salmon, the workshop covers best practice guidelines for RNA-seq experimental design and data organization/management.

Learning Objectives

• Use the command line interface (bash) and HPC to analyze high-throughput sequencing data
• Implement best practices for designing an RNA-seq experiment and analysis the resulting data