Skip to content

igemsoftware2020/igem_epfl_2020

Folders and files

NameName
Last commit message
Last commit date

Latest commit

 

History

32 Commits
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Repository files navigation

iGEM 2020 - EPFL team's Github

This repository has the necessary code, CAD and circuit files in order to build your own ExpressEAU.

Project Abstract

Water is an essential resource that we regularly use for drinking, cooking and food processing. Hence, it is important to ensure that it is safe for consumption. EspressEAU serves as an analysis of general water quality. We aimed to create a safe, low-cost, and easy-to-use system that can enable on-site testing of water at home or in small communities. We hypothesized that genetically engineered yeast could be used as biosentinels by hijacking the yeast stress response pathway using either deletion strains or fluorescent reporter strains that are sensitive to water contaminants. These biosentinels are grown in a DIY system with temperature control, stirring, and two optical detectors for fluorescence and density, allowing us to monitor growth and fluorescence of the yeast, and thus detect the presence of contaminants in the water sample. We hope our project will facilitate frequent water testing of local water sources.

Software used

  1. Tinkercad: Used to make the CAD designs for 3D print, using STL files (https://www.tinkercad.com/)
  2. Circuit Diagram: Used to make circuit schematics using an online IDE (https://www.circuit-diagram.org/)
  3. PRUSA 3D Slicer: Used to generate the G Code and the sliced version of the 3D element (https://www.prusa3d.com/drivers/)
  4. Processing: Used to interface between the Arduino Serial Interface and to collect data on the host machine (https://processing.org/)

Hardware Components used

Microcontroller: Arduino Uno

Arduino_Uno

Source

Cable: USB for data transfer

USB cable

Datasheet
Source

Temperature Sensor: TMP36

TMP36

Datasheet
Source

Light to Frequency Sensor: TSL235R (primarily for optical density measurement)

light_to_frequency

Datasheet
Source

RGB Light Sensor: ISL9125 (primarily for fluorescence measurement)

TMP36

Datasheet
Source

OEM Temperature sensor: DS18B20 (for calibration and check of TMP36 sensor)

DS18B20

Datasheet
Source

Power Supply 9V, 1.5A (Variable): 3 V - 12 V universal power supply 1.5A

TMP36

Source

Thermoelectric Peltier Element: TEC1-12706

TEC1-12706

Datasheet
Source

IRF520 Mosfet Transistor

ESC

Datasheet
Source

DC Motor (working with 6V or 9V - 9V is generally more consistent)

DCMotor_

Datasheet
Source

Pack of LEDs - white LED and Orange LED (with wave length between 530-560 nm for fluorescence measurement)

Battery 9V

Jumper wires (types: Male-Female, Female-Female, Male-Male)

Soldering iron with tin

Glue gun (with glue sticks)

About

iGEM 2020 CAD and code files

Resources

Stars

Watchers

Forks

Releases

No releases published

Packages

No packages published