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@insights-engineering-bot insights-engineering-bot released this 19 Aug 22:13
  • First release of the hermes package, which contains classes, methods and functions to import, quality-check, filter, normalize, and analyze RNAseq counts data for differential expression.
  • hermes is a successor of the rnaseqTools R package. The core functionality is built on the BioConductor ecosystem, especially the SummarizedExperiment class. New users should first begin by reading the "Introduction to hermes" vignette to become familiar with the hermes concepts.
  • Import RNAseq count data into the hermes ready format.
  • Annotate gene information from the Ensembl database via biomaRt.
  • Add quality control (QC) flags to genes and samples.
  • Filter and subset the data set.
  • Normalize the counts.
  • Produce descriptive plots.
  • Perform principal components analysis.
  • Produce a templated QC Rmd report.
  • Perform differential expression analysis.