Data analysis
DBCLS SRA Metadata toolkit project provides results of FastQC, one of the most popular quality control software for high-throughput sequencing.
Reasons that we built this are:
- Failed sequencing data are also submitted
- Due to grant restriction or other reasons
- Data download requires long time, even if the file is broken
- Number of read, GC content, and some other information is necessary for further analysis
- Users perform QC before starting analysis anyway
Users will perform further analysis on their analysis pipeline and computer platform. ENA and DRA also provide analysis platform for public data.
"DDBJ Read Annotation Pipeline is a cloud-computing based analytical platform for next-generation sequencing data."
Users can upload their local data files to perform analysis on the DDBJ super-computing platform, and also use DRA data. DDBJ also provide galaxy platform for secondary analysis. Provided primary analysis tools are as follows:
Reference Genome Mapping Tools
- BLAT
- Maq
- bwa
- SOAP
- Bowtie
- TopHat
- Bowtie2
de novo Assembly
- SOAPdenovo
- ABySS
- Velvet
- Trinity
Mapping Contigs by de novo Assemble to Reference Sequences
- BLAT
EBI provides the feature importing files to galaxy pipeline. Click "Fastq files (galaxy)" in the table on result page to import and do further analysis.