Snakemake workflow

Title: Jez cloning test

Bulk RNA-Seq Telescope analysis of 2 metastatic melanoma datasets. Input the metadata table that include the accessions needed to download the raw sequencing files, output gene/HERV count matrices.

Dataset Citations

1. Hugo, W., Zaretsky, J. M., Sun, L. U., Song, C., Moreno, B. H., Hu-Lieskovan, S., ... & Lo, R. S. (2016). Genomic and transcriptomic features of response to anti-PD-1 therapy in metastatic melanoma. Cell, 165(1), 35-44.

2. Van Allen, E. M., Miao, D., Schilling, B., Shukla, S. A., Blank, C., Zimmer, L., ... & Garraway, L. A. (2015). Genomic correlates of response to CTLA-4 blockade in metastatic melanoma. Science, 350(6257), 207-211.

Workflow Graphs

To get DAG:

snakemake --profile profiles/aws --forceall --dag | dot -Tpdf > dag.pdf

To get rule graph:

snakemake --profile profiles/aws --forceall --rulegraph | dot -Tpdf > rulegraph.pdf

To get file graph:

snakemake --profile profiles/aws --forceall --filegraph | dot -Tpdf > filegraph.pdf

To run pipeline:

snakemake --profile profiles/aws/ all

To modify pipeline:

Change sample download table and method. This pipeline uses prefetchthenfastq-dump to download files.

Usage

If you use this workflow in a paper, don't forget to give credits to the authors by citing the URL of this (original) repository: https://github.com/nixonlab/Mets_CM_bulk