Skip to content

johnegarza/chromoseq

Folders and files

NameName
Last commit message
Last commit date

Latest commit

 

History

64 Commits
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Repository files navigation

Files Chromoseq.v9.wdl, application.new.conf, and LaunchChromoSeqFromCram.sh are for running locally (LSF) at MGI

Chromoseq_basespace.v9.wdl has all runtime attributes stripped, since the basespace cromwell workflow currently operates on a single node and is not configured to launch each job in a new container (this would waste resources- the current container already has everything each task needs to run)

launch_and_monitor_chromoseq.pl is for launching basespace from MGI

Instructions on running basespace and other information is written in https://confluence.ris.wustl.edu/display/CI/Basespace+SOP

forms & callbacks.js are configuration files from basespace that control the app input parameters and the command line instructions used to launch the app- these must be manually updated
current versions were last modified on 4/4/19
these can be accessed by logging in to the MGI organization and going to https://developer.basespace.illumina.com/apps/6984978/forms/revisions


launch_and_monitor_chromoseq.pl: finding constants

RefId
This was originally generated in project full_workflow_test1, analysis DRAGEN Reference Builder 03/28/2019 10:51:57
Analysis url: https://basespace.illumina.com/analyses/170755135?projectId=123592472
bs appsession get -i 170755135
bs list appresults --project-name=full_workflow_test1 --filter-field=AppSession.Id --filter-term=170755135
+-----------+---------------+----------------------------------------------+
|    Id     |     Name      |               AppSession.Name                |
+-----------+---------------+----------------------------------------------+
| 213691478 | all_sequences | DRAGEN Reference Builder 03/28/2019 10:51:57 |
+-----------+---------------+----------------------------------------------+
bs content appresult --id=213691478
+-------------+----------------------+
|     Id      |       FilePath       |
+-------------+----------------------+
| 14321367413 | all_sequences.v7.tar |
+-------------+----------------------+
The above ID is the value used in the workflow

ref_fasta
https://basespace.illumina.com/analyses/173645472?projectId=123592472
bs list appresults --project-name=full_workflow_test1 --filter-field=AppSession.Id --filter-term=173645472
+-----------+---------------+-----------------+
|    Id     |     Name      | AppSession.Name |
+-----------+---------------+-----------------+
| 216723517 | all_sequences | all_sequences   |
+-----------+---------------+-----------------+
bs content appresult --id=216723517
+-------------+------------------+
|     Id      |     FilePath     |
+-------------+------------------+
| 14477433053 | all_sequences.fa |
+-------------+------------------+