This is a project to determine whether the insectary environment affects mosquito microbiome composition.
A batch of Aedes aegypti eggs were split and reared concurrently in three different insectaries at Liverpool School of Tropical Medicine. 16S rRNA data was then generated from mosquitoes at different life stages as well as from their environment and food sources and environemtnal conditions were also measured. The data were then compared across the three insectaries.
Brettell, L.E., Hoque, A.F., Joseph, T.S., Dhokiya, V., Hornett, E.A., Hughes, G.L. and Heinz, E., 2024. Mosquitoes reared in distinct insectaries within an institution in close spatial proximity possess significantly divergent microbiomes.
The manuscript is currently on biorxiv doi: https://doi.org/10.1101/2024.08.28.610121. However, it has now been peer reviewed and accepted for publication and the doi will be updated when available.
The directories Supp_figs and Supp_tables contain all the Supplementary figures and tables referenced in the manuscript. Each directory also contains a file containing all the relevant figure and table legends.
The directory Scripts_and_required_files contains all scripts used to analyse data and generate figures as well as all the required data files. Scripts and code were generated by LEB, VD and EH.
The raw data (sequencing reads) for this project are publicly available at Sequence Read Archive (SRA) under project code PRNJ1115112 and accession numbers for each sample are given in Table S4.