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| name: Deploy Gitbuilding Project to Github Pages | ||
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| <!DOCTYPE html> | ||
| <html> | ||
| <head> | ||
| <meta charset="utf-8"> | ||
| <title>Strobe-enhanced microscopy stage</title> | ||
| <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
| <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
| <link rel="shortcut icon" href="./assets/favicon.ico" /> | ||
|
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| <link href="./static/style.css" rel="stylesheet"> | ||
| <link href="./assets/style.css" rel="stylesheet"> | ||
|
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| </head> | ||
| <body> | ||
| <header class="site-header"> | ||
| <div class="wrapper header-wrapper" > | ||
| <div class=header-text> | ||
|
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| <h1 class="site-title">Strobe-enhanced microscopy stage</h1> | ||
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| <p class="author">by Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p> | ||
| <p class="affiliation">Latin American Hub for Bioimaging Through Open Hardware (LIBRE hub)</p> | ||
| </div> | ||
| </div> | ||
| </header> | ||
| <div class="page-content"> | ||
| <div> | ||
| <nav class="sidebar"> | ||
| <a href="#" class="menu-icon"> | ||
| <svg viewBox="0 0 18 15"> | ||
| <path fill="#424242" d="M18,1.484c0,0.82-0.665,1.484-1.484,1.484H1.484C0.665,2.969,0,2.304,0,1.484l0,0C0,0.665,0.665,0,1.484,0 h15.031C17.335,0,18,0.665,18,1.484L18,1.484z"/> | ||
| <path fill="#424242" d="M18,7.516C18,8.335,17.335,9,16.516,9H1.484C0.665,9,0,8.335,0,7.516l0,0c0-0.82,0.665-1.484,1.484-1.484 h15.031C17.335,6.031,18,6.696,18,7.516L18,7.516z"/> | ||
| <path fill="#424242" d="M18,13.516C18,14.335,17.335,15,16.516,15H1.484C0.665,15,0,14.335,0,13.516l0,0 c0-0.82,0.665-1.484,1.484-1.484h15.031C17.335,12.031,18,12.696,18,13.516L18,13.516z"/> | ||
| </svg> | ||
| </a> | ||
| <ul class="nav-list"> | ||
| <li> | ||
| <a class="navhome" href="./"> | ||
| Strobe-enhanced microscopy stage | ||
| </a> | ||
| </li> | ||
| <li class="active"> | ||
| <a class="active" href="./3-level-station.html"> | ||
| Build the 3-level microscopy stage | ||
| </a> | ||
| <ul class="sub-nav-list"> | ||
| <li> | ||
| <a class="not-active" href="./printing.html"> | ||
| Print the plastic parts | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./laser-cutting.html"> | ||
| Laser cut the acrylic parts | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./assemble-focus-mechanism.html"> | ||
| Assemble the focus mechanism | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./basic-optics-module.html"> | ||
| Assemble the basics optics module | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./attach-parts-to-top-plate.html"> | ||
| Attach parts to the top plate | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./attach-parts-to-middle-plate.html"> | ||
| Attach parts to the middle plate | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./attach-parts-to-bottom-plate.html"> | ||
| Attach parts to the bottom plate | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./mount-optics.html"> | ||
| Mount the optics | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./wiring.html"> | ||
| Mount the electronics | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./illumination.html"> | ||
| Assemble the illumination | ||
| </a> | ||
| </li> | ||
| <li> | ||
| <a class="not-active" href="./finished.html"> | ||
| Assembly complete | ||
| </a> | ||
| </li> | ||
| </ul> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./software/software-installation.html"> | ||
| Software Installation | ||
| </a> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./usage.html"> | ||
| Use your Stage | ||
| </a> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./troubleshooting.html"> | ||
| Troubleshooting | ||
| </a> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./variants_accesories.html"> | ||
| Customisation and accessories | ||
| </a> | ||
| </li> | ||
|
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||
| </ul> | ||
| </nav> | ||
| </div> | ||
| <div class="wrapper"> | ||
| <h1 id="3-level-microscopy-stage">3-level microscopy stage</h1> | ||
| <p><img alt="" src="images/3-level-station.jpg" title="" /></p> | ||
| <p>Before you start building the station, you will need to source all the components listed in our <a href="3-level-station_BOM.html" title="HTML">bill of materials</a> (<a href="3-level-station_BOM.html"><img alt="HTML" class="smallicon" src="static/Icons/html.png" title="HTML Bill of Materials" /></a>, <a href="3-level-station_BOM.csv"><img alt="CSV" class="smallicon" src="static/Icons/csv.png" title="CSV Bill of Materials" /></a>), which is given on the next page.</p> | ||
| <h2 id="instructions">Instructions</h2> | ||
| <p>These instructions will take you through how to assemble the 3-level version of the stage. For more designs, go to <a href="variants_accesories.html" title="">Customisation and accessories</a>.</p> | ||
| <p>The assembly is described in several steps:</p> | ||
| <ol> | ||
| <li><a href="printing.html" title="">Print the plastic parts</a></li> | ||
| <li><a href="laser-cutting.html" title="">Laser cut the acrylic parts</a></li> | ||
| <li><a href="assemble-focus-mechanism.html" title="">Assemble the focus mechanism</a></li> | ||
| <li><a href="basic-optics-module.html" title="">Assemble the basic optics module</a></li> | ||
| <li><a href="attach-parts-to-top-plate.html" title="">Attach parts to the top plate</a></li> | ||
| <li><a href="attach-parts-to-middle-plate.html" title="">Attach parts to the middle plate</a></li> | ||
| <li><a href="attach-parts-to-bottom-plate.html" title="">Attach parts to the bottom plate</a></li> | ||
| <li><a href="mount-optics.html" title="">Mount the optics</a></li> | ||
| <li><a href="wiring.html" title="">Mount the electronics</a></li> | ||
| <li><a href="illumination.html" title="">Assemble the illumination</a></li> | ||
| <li><a href="finished.html" title="">Assembly complete</a></li> | ||
| </ol> | ||
| <hr /> | ||
| <p><a href="3-level-station_BOM.html">Next page</a></p> | ||
| </div> | ||
| </div> | ||
| <footer class="site-footer"> | ||
| <div class="wrapper"> | ||
| <a target="_blank" rel="noopener noreferrer" href="https://gitbuilding.io"> | ||
| <img class="icon" src="./static/Logo/GitBuilding500x.png" alt="GitBuilding logo"> | ||
| <span class="info">Documentation powered by GitBuilding</span> | ||
| </a> | ||
| <p class="author">©2023 Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p> | ||
| <p class="email">Contact: <a href="mailto:librehub@uc.cl">librehub@uc.cl</a></p> | ||
| <p class="license">Strobe-enhanced microscopy stage is released under <a href="license.html">CERN-OHL-S-2.0</a>.</p> | ||
|
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| </div> | ||
| </footer></body> | ||
| </html> |
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| # 3-level microscopy stage | ||
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|  | ||
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| Before you start building the station, you will need to source all the components listed in our [bill of materials]{BOM}, which is given on the next page. | ||
|
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| ## Instructions | ||
|
|
||
| These instructions will take you through how to assemble the 3-level version of the stage. For more designs, go to [Customisation and accessories](variants_accesories.md). | ||
|
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| The assembly is described in several steps: | ||
|
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| 1. [.](printing.md){step} | ||
| 1. [.](laser-cutting.md){step} | ||
| 1. [.](assemble-focus-mechanism.md){step} | ||
| 1. [.](basic-optics-module.md){step} | ||
| 1. [.](attach-parts-to-top-plate.md){step} | ||
| 1. [.](attach-parts-to-middle-plate.md){step} | ||
| 1. [.](attach-parts-to-bottom-plate.md){step} | ||
| 1. [.](mount-optics.md){step} | ||
| 1. [.](wiring.md){step} | ||
| 1. [.](illumination.md){step} | ||
| 1. [.](finished.md){step} |
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| Name in documentation Category Qty Full Name Notes | ||
| #1 pozidrive screwdriver tool 1 | ||
| 1.5mm Ball-end Allen key tool 1 | ||
| 2 pin Du Pont connector female housing electronic 1 | ||
| 2.5mm Ball-end Allen key tool 1 | ||
| 30x30x10mm Heat sink electronic 1 | ||
| 52x18x0.5cm Acrylic sheet material 1 | ||
| Black PLA filament material 50 g | ||
| Conductor Flexible Cables electronic 2 | ||
| Crimper tool 1 | ||
| Double-sided self adhesive tape electronic 1 | ||
| Double-sided thermal tape electronic 1 | ||
| Heat insert mechanic 7 Heat inserts | ||
| High-power star LED electronic 1 | ||
| Laser cutting machine tool 1 | ||
| light oil consumable 1 drop Optional | ||
| M2x5mm cap head screw mechanic 4 Screw | ||
| M3 nut mechanic 14 | ||
| M3 washers mechanic 2 Screw washer | ||
| M3x10mm cap head screw mechanic 11 Screw | ||
| M3x15mm cap head screw mechanic 8 Screw | ||
| M3x20mm pozi pan head screw mechanic 1 M3 X Screw | ||
| M3x25mm cap head screw mechanic 4 Screw | ||
| M3x25mm hex head screw mechanic 1 M3 HEX screw | ||
| M3x8mm cap head screw mechanic 2 Screw | ||
| Male Crimp Pin electronic 2 | ||
| Needle-nose plier tool 1 | ||
| Nitrile gloves consumable 2 | ||
| O-ring mechanic 1 | ||
| Pi Camera lens tool tool 1 This should come with the Raspberry Pi Camera Module. If it is missing, you can 3D print a workaround lens remover. | ||
| Pi camera ribbon cable electronic 1 | ||
| Pi HAT electronic 1 This is a custom open-source board documented [here](https://github.com/wenzel-lab/open-microfluidics-workstation/) | ||
| PLA filament material 205 g Of any colour you want | ||
| Precision wire cutter tool 1 | ||
| Raspberry Pi electronic 1 | ||
| Raspberry Pi Camera Module v2 electronic 1 | ||
| Raspberry Pi Power Supply electronic 1 | ||
| RepRap-style printer tool 1 | ||
| Soldering iron tool 1 | ||
| Star-LED lens optical 1 | ||
| Strobe Cable electronic 1 This is a custom connector documented [here](https://github.com/wenzel-lab/open-microfluidics-workstation/) | ||
| Strobe Module electronic 1 This is a custom open-source board documented [here](https://github.com/wenzel-lab/open-microfluidics-workstation/) | ||
| Strobe Power Supply electronic 1 Strobe Power Supply | ||
| Utility knife tool 1 Not a scalpel! | ||
| Voltage regulator electronic 1 This regulator has modifications documented [here](https://github.com/wenzel-lab/open-microfluidics-workstation/) |
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| @@ -0,0 +1,152 @@ | ||
| <!DOCTYPE html> | ||
| <html> | ||
| <head> | ||
| <meta charset="utf-8"> | ||
| <title>Strobe-enhanced microscopy stage</title> | ||
| <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
| <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
| <link rel="shortcut icon" href="./assets/favicon.ico" /> | ||
|
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||
| <link href="./static/style.css" rel="stylesheet"> | ||
| <link href="./assets/style.css" rel="stylesheet"> | ||
|
|
||
| </head> | ||
| <body> | ||
| <header class="site-header"> | ||
| <div class="wrapper header-wrapper" > | ||
| <div class=header-text> | ||
|
|
||
| <h1 class="site-title">Strobe-enhanced microscopy stage</h1> | ||
|
|
||
| <p class="author">by Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p> | ||
| <p class="affiliation">Latin American Hub for Bioimaging Through Open Hardware (LIBRE hub)</p> | ||
| </div> | ||
| </div> | ||
| </header> | ||
| <div class="page-content"> | ||
| <div> | ||
| <nav class="sidebar"> | ||
| <a href="#" class="menu-icon"> | ||
| <svg viewBox="0 0 18 15"> | ||
| <path fill="#424242" d="M18,1.484c0,0.82-0.665,1.484-1.484,1.484H1.484C0.665,2.969,0,2.304,0,1.484l0,0C0,0.665,0.665,0,1.484,0 h15.031C17.335,0,18,0.665,18,1.484L18,1.484z"/> | ||
| <path fill="#424242" d="M18,7.516C18,8.335,17.335,9,16.516,9H1.484C0.665,9,0,8.335,0,7.516l0,0c0-0.82,0.665-1.484,1.484-1.484 h15.031C17.335,6.031,18,6.696,18,7.516L18,7.516z"/> | ||
| <path fill="#424242" d="M18,13.516C18,14.335,17.335,15,16.516,15H1.484C0.665,15,0,14.335,0,13.516l0,0 c0-0.82,0.665-1.484,1.484-1.484h15.031C17.335,12.031,18,12.696,18,13.516L18,13.516z"/> | ||
| </svg> | ||
| </a> | ||
| <ul class="nav-list"> | ||
| <li> | ||
| <a class="navhome" href="./"> | ||
| Strobe-enhanced microscopy stage | ||
| </a> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./3-level-station.html"> | ||
| Build the 3-level microscopy stage | ||
| </a> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./software/software-installation.html"> | ||
| Software Installation | ||
| </a> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./usage.html"> | ||
| Use your Stage | ||
| </a> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./troubleshooting.html"> | ||
| Troubleshooting | ||
| </a> | ||
| </li> | ||
| <li class="not-active"> | ||
| <a class="not-active" href="./variants_accesories.html"> | ||
| Customisation and accessories | ||
| </a> | ||
| </li> | ||
|
|
||
| </ul> | ||
| </nav> | ||
| </div> | ||
| <div class="wrapper"> | ||
| <h1 id="bill-of-materials">Bill of Materials</h1> | ||
| <p>Download this as a <a href="3-level-station_BOM.csv">CSV file</a></p> | ||
| <h3 id="tool">Tools</h3> | ||
| <ul> | ||
| <li>1 <a Class="bom" href="parts/tools/screwdriver.html">#1 pozidrive screwdriver</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/1.5mmBallEndAllenKey.html">1.5mm Ball-end Allen key</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/2.5mmBallEndAllenKey.html">2.5mm Ball-end Allen key</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/crimper.html">Crimper</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/laser-cutting-machine.html">Laser cutting machine</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/pliers.html">Needle-nose plier</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/pi_camera_lens_tool.html">Pi Camera lens tool</a> - This should come with the Raspberry Pi Camera Module. If it is missing, you can 3D print a workaround lens remover.</li> | ||
| <li>1 <a Class="bom" href="parts/tools/precision-wire-cutters.html">Precision wire cutter</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/rep-rap.html">RepRap-style printer</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/soldiron.html">Soldering iron</a> </li> | ||
| <li>1 <a Class="bom" href="parts/tools/utility-knife.html">Utility knife</a> - Not a scalpel!</li> | ||
| </ul> | ||
| <h3 id="consumable">Consumables</h3> | ||
| <ul> | ||
| <li>1 drop of <a Class="bom" href="parts/consumables/light-oil.html">light oil</a> - Optional</li> | ||
| <li>2 <a Class="bom" href="parts/consumables/gloves.html">Nitrile gloves</a> </li> | ||
| </ul> | ||
| <h3 id="electronic">Electronic Components</h3> | ||
| <ul> | ||
| <li>1 <a Class="bom" href="parts/elect/2pin-DuPont-female-housing.html">2 pin Du Pont connector female housing</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/30x30x10mm-heatsink.html">30x30x10mm Heat sink</a> </li> | ||
| <li>2 <a Class="bom" href="parts/elect/protoboard-cables.html">Conductor Flexible Cables</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/double-sided-adhesive-tape.html">Double-sided self adhesive tape</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/double-sided-thermal-tape.html">Double-sided thermal tape</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/high-power-star-LED.html">High-power star LED</a> </li> | ||
| <li>2 <a Class="bom" href="parts/elect/male-crimp-pin.html">Male Crimp Pin</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/rpi-camera-ribbon-cable.html">Pi camera ribbon cable</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/pi-hat.html">Pi HAT</a> - This is a custom open-source board documented <a href="https://github.com/wenzel-lab/open-microfluidics-workstation/" target="_blank" title="">here</a></li> | ||
| <li>1 <a Class="bom" href="parts/elect/rpi-v4.html">Raspberry Pi</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/rpi-camera-v2.html">Raspberry Pi Camera Module v2</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/rpi-power-supply.html">Raspberry Pi Power Supply</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/strobe-cable.html">Strobe Cable</a> - This is a custom connector documented <a href="https://github.com/wenzel-lab/open-microfluidics-workstation/" target="_blank" title="">here</a></li> | ||
| <li>1 <a Class="bom" href="parts/elect/strobe-module.html">Strobe Module</a> - This is a custom open-source board documented <a href="https://github.com/wenzel-lab/open-microfluidics-workstation/" target="_blank" title="">here</a></li> | ||
| <li>1 <a Class="bom" href="parts/elect/strobe-power-supply.html">Strobe Power Supply</a> </li> | ||
| <li>1 <a Class="bom" href="parts/elect/voltage-regulator.html">Voltage regulator</a> - This regulator has modifications documented <a href="https://github.com/wenzel-lab/open-microfluidics-workstation/" target="_blank" title="">here</a></li> | ||
| </ul> | ||
| <h3 id="material">Materials</h3> | ||
| <ul> | ||
| <li>1 <a Class="bom" href="parts/materials/acrylic-sheet.html">52x18x0.5cm Acrylic sheet</a> </li> | ||
| <li>50 g of <a Class="bom" href="parts/materials/black_pla_filament.html">Black PLA filament</a> </li> | ||
| <li>205 g of <a Class="bom" href="parts/materials/pla_filament.html">PLA filament</a> - Of any colour you want</li> | ||
| </ul> | ||
| <h3 id="mechanic">Mechanical Components</h3> | ||
| <ul> | ||
| <li>7 <a Class="bom" href="parts/mech/Hinsert.html">Heat insert</a> </li> | ||
| <li>4 <a Class="bom" href="parts/mech/M2-5.html">M2x5mm cap head screw</a> </li> | ||
| <li>14 <a Class="bom" href="parts/mech/nuts.html">M3 nut</a> </li> | ||
| <li>2 <a Class="bom" href="parts/mech/washer.html">M3 washers</a> </li> | ||
| <li>11 <a Class="bom" href="parts/mech/M3-10.html">M3x10mm cap head screw</a> </li> | ||
| <li>8 <a Class="bom" href="parts/mech/M3-15.html">M3x15mm cap head screw</a> </li> | ||
| <li>1 <a Class="bom" href="parts/mech/X-M3-20.html">M3x20mm pozi pan head screw</a> </li> | ||
| <li>4 <a Class="bom" href="parts/mech/M3-25.html">M3x25mm cap head screw</a> </li> | ||
| <li>1 <a Class="bom" href="parts/mech/HEX-M3-25.html">M3x25mm hex head screw</a> </li> | ||
| <li>2 <a Class="bom" href="parts/mech/M3-8.html">M3x8mm cap head screw</a> </li> | ||
| <li>1 <a Class="bom" href="parts/mech/oring.html">O-ring</a> </li> | ||
| </ul> | ||
| <h3 id="optical">Optical Components</h3> | ||
| <ul> | ||
| <li>1 <a Class="bom" href="parts/optics/8.7-20mm-circular-lens.html">Star-LED lens</a> </li> | ||
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