in-frame p-site quantification method #141
Open
+151
−4
Add this suggestion to a batch that can be applied as a single commit.
This suggestion is invalid because no changes were made to the code.
Suggestions cannot be applied while the pull request is closed.
Suggestions cannot be applied while viewing a subset of changes.
Only one suggestion per line can be applied in a batch.
Add this suggestion to a batch that can be applied as a single commit.
Applying suggestions on deleted lines is not supported.
You must change the existing code in this line in order to create a valid suggestion.
Outdated suggestions cannot be applied.
This suggestion has been applied or marked resolved.
Suggestions cannot be applied from pending reviews.
Suggestions cannot be applied on multi-line comments.
Suggestions cannot be applied while the pull request is queued to merge.
Suggestion cannot be applied right now. Please check back later.
This PR adds another (experimental) quantification method for Ribo-seq data. Instead of counting all Ribo-seq reads which map to a transcript, this new method only counts reads which map to coding regions (
CDS) and only if the P-sites computed by Plastid align with the reading frame of the coding region. This means the quantification method is fairly conservative, ignoring frame-shifted reads or reads mapping to unannotated coding regions. RNA-seq quantification is unchanged.The user can enable this quantification method by setting
--te_quantification_methodto the valueplastid_psite. It is off by default. The method is implemented as a local module (essentiallybedtools intersect).I meant to add a test for this new quantification method, but the inputs to the module are not deterministic to begin with which means the outputs aren't either, making a test rather pointless.
PR checklist
nf-core pipelines lint).nextflow run . -profile test,docker --outdir <OUTDIR>).nextflow run . -profile debug,test,docker --outdir <OUTDIR>).docs/usage.mdis updated.docs/output.mdis updated.CHANGELOG.mdis updated.README.mdis updated (including new tool citations and authors/contributors).