harpy v0.6.0
Changed behavior
Instead of using sambamba markdups
, harpy now uses samtools markdup -S --barcode-tag BX
, which does two great things:
- uses linked-read barcode information in assessing PCR duplicates
- marks supplementary (chimeric) reads as duplicates if the primary alignment is marked as a duplicate
What's Changed
Full Changelog: 0.5.0...0.6.0