1.12
What's new (and important)
simulate linkedreads
now supports and defaults to haplotagging barcodes
- 84 million barcode options instead of 14m
- support for barcodes of any length, not just the 10X 16bp
- barcode sequencing error has been removed because you're ultimately interested in the linked read data, not the sequencing nuances
Internal
HaploSim.pl
(formerly LRSIM_harpy.pl) focuses solely on creating linked reads from provided haplotypes- output names for
simulate linkedreads
more flexible now - leveraged parameters better in
HaploSim.pl
- Added
haplotag_barcodes.py
to auto-generate haplotag barcodes - inline to haplotagging conversion uses memory-efficient in-memory sqlite3 database
- barcode validations for
align ema
andsimulate linkedreads
Bugs fixed
- [simulate linkedreads] barcode key generated as a fixed keymap, ensuring barcodes have same haplotag code between different haplotypes
What's Changed
- haplotagging barcodes as default by @pdimens in #162
- better sim demux support by @pdimens in #163
- fix param call by @pdimens in #164
Full Changelog: 1.11...1.12