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New
added a convenience script separate_singletons to split a bam file into singletons and nonsingletons
harpy downsample module to downsample FASTQ/BAM by barcodes
Breaking changes
singletons are now calculated such that both reads of a paired-end read only counts as "one read" for a barcode
which means unpaired reads now contribute properly to this value
overall, this is a more accurate way of calculating this metric
Fixes
separate_validbx has a usage change, which is breaking, however this script is not used by any of the workflows so there should be no appreciable difference
alignment reports have text that clarifies which math is for non-singletons
multiplex reads (aka reads that arent linked-read singletons) are now just referred to as non-singletons