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1.14

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@github-actions github-actions released this 06 Dec 21:42
· 17 commits to main since this release
e1867c2

New

  • added a convenience script separate_singletons to split a bam file into singletons and nonsingletons
  • harpy downsample module to downsample FASTQ/BAM by barcodes

Breaking changes

  • singletons are now calculated such that both reads of a paired-end read only counts as "one read" for a barcode
    • which means unpaired reads now contribute properly to this value
    • overall, this is a more accurate way of calculating this metric

Fixes

  • separate_validbx has a usage change, which is breaking, however this script is not used by any of the workflows so there should be no appreciable difference
  • alignment reports have text that clarifies which math is for non-singletons
  • multiplex reads (aka reads that arent linked-read singletons) are now just referred to as non-singletons