Long Surrey
New Features
- fastq_to_bam implementation in #219
If the parameter --bam_output is provided, CRISPResso alignments will be written to a file called 'CRISPResso_output.bam' with the alignments in bam format. If thebowtie2_index
is provided, alignments will be reported in reference to that genome. If thebowtie2_index
is not provided, alignments will be reported in reference to a custom reference created by the amplicon sequence(s) and written to the file 'CRISPResso_output.fa'.
This enables the viewing of CRISPResso alignments in other browsers (e.g., IGV). If nobowtie2_index
is provided, the reference genome should be set to the produced 'CRISPResso_output.fa' file, and then the alignment bam can be loaded into IGV.
Minor Fixes
- CRISPRessobatch: put directory in quotes by @sshen8 in #222
- Don't run global frameshift plot when there are no modified reads by @Colelyman in #226
Full Changelog: v2.2.8...v2.2.9