Nanorods
Nanorods samples were wet, so the background of images is inhomogenous.
Some nanorods also contain bright parts, so there is problem with segmentation, because algorithm divides it into more particles.
These particles can be eliminated, so the algorithm does not take them in account.
There is also problem with watershed, which oversegments particles or is not able to divide them well. This problem was solved with repeated erosion (ultimate erosion), which gives illegible seeds for watershed. There us also problem with bright parts in some nanorods. These nanorods were also oversegmented, so program detects them and removes them from evaluation.
