Code for Comprehensive Analysis of Alternative Splicing Across Tumors from 8,705 Patients

Public repository containing research code for the TCGA PanCanAtlas Splicing project accompanying the manuscript Comprehensive Analysis of Alternative Splicing Across Tumors from 8,705 Patients

The code is organized in several sub-directories, which contain the following content:

• alt_splice Code concerning the detection and analysis of alternative splicing events.

  • events
    Wrapper code for the detection of splicing events in the cohort using SplAdder.

  • event_stats
    Code for visualising the event type distributions and number of alternative splicing events detected per cancer type.

  • filtering
    Code implementing various filters on the AS events, based on read coverage, min delta PSI, etc.

  • complexity
    Detection of neo-junctions predominantly present in cancer samples but absent from annotation or the GTEx outgroup.

  • splice_outliers
    Code for detection and visualization of splicing outliers.

  • tumor_vs_normal_rank
    Identification and ranking of tumor specific introns.

  • tumor_vs_normal_stats
    Assessment of events detected in tumor samples vs normal samples.

  • tumor_vs_normal_test
    Differential testing of tumor vs normal samples on sets of subsamples per cancer type.

• neoepitopes
  Contains code for the visualization of results on the prediction of neo-epitopes.

• peptide_generation
  Code concerned with generating peptides from splice-graphs.

• qc
  Code and additional resources related to quality control of input data.

• sample_processing_rna
  Code for pre-processing and alignment of the RNA-Seq data, including expression counting, the collection of usage statistics, and construction of splicing graphs.

• sqtl
  Visualization scripts for the sQTL analyses.

• tSNE_and_heatmap_visualizations
  Code for tSNE embeddings on alternative splicing and expression data as well as scripts for visualization and different highlightings.