Few True Effects

• RNA-seq data was simulated with polyester R package (Frazee, 2015) on 20,000 transcripts from human transcriptome using grid of 3, 6, and 10 replicates and 100, 200, 400, and 800 effects for two groups. Fold changes were set to 0.5 and 2.
• Differential expression was assessed using DESeq2 R package (Love, 2014) using default settings and group 1 versus group 2 contrast.