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project planning post update
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58 changes: 29 additions & 29 deletions docs/index.html
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Expand Up @@ -234,7 +234,7 @@ <h3 class="no-anchor listing-title">
<a href="./posts/projects/coral-pae-temp.html"> </a>
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Experimental tank setup
Daily Lab Log
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Enter at your peril; This one is for me, not you
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protocols
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anemones
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daily log
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Hydra 32HD manual
Lab meeting cancelled
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<div class="listing-date">
Feb 9, 2024
Feb 21, 2024
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Sarah Tanja
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<a href="./posts/projects/anemone/tanks/tanks.html">
<h3 class="no-anchor listing-title">
Daily Lab Log
Experimental tank setup
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Enter at your peril; This one is for me, not you

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<div class="listing-category" onclick="window.quartoListingCategory('daily log'); return false;">
daily log
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protocols
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anemones
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<a href="./posts/daily-log/index.html">
<a href="./posts/projects/anemone/tanks/tanks.html">
<div class="listing-description">
Lab meeting cancelled
Hydra 32HD manual
</div>
</a>
</div>
<div class="metadata">
<a href="./posts/daily-log/index.html">
<a href="./posts/projects/anemone/tanks/tanks.html">
<div class="listing-date">
Feb 8, 2024
Feb 9, 2024
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<div class="listing-author">
Sarah Tanja
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<a href="./posts/projects/anemone/project-planning.html">
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::: callout-note Overarching question Does chronic microplastic-associated leachate pollution reduce marine anthozoan’s climate resilience to marine heatwaves? :::
Does chronic microplastic-associated leachate pollution reduce Cnidarian’s resilience to marine heatwaves?
</div>
</a>
</div>
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Expand Up @@ -215,7 +215,7 @@ <h1 class="title">Anthopleura elegantissima</h1>
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&nbsp;
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2 min
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<img src="../../posts/projects/anemone/photosynth-pam/thumbnail.jpg" style="height: 40px;">
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learning journey,anemones
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2 min
3 min
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3 changes: 2 additions & 1 deletion docs/posts/projects/anemone/about-anemones/about-anemone.html
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Expand Up @@ -183,6 +183,7 @@ <h1 class="title">The aggregating anemone, Anthopleura elegantissima</h1>


<p><em>Anthopleura elegantissima</em></p>
<p>Puget Sound Museum of Natural History article <a href="https://www.pugetsound.edu/puget-sound-museum-natural-history/exhibits/marine-panel/aggregating-anemone">here</a></p>
<blockquote class="blockquote page-columns page-full">
<p>“This anemone is an effective predator living in zooplankton-rich water, so one would not expect it to be as nutrient-limited, nor as dependent on algal photosynthetic productivity as its tropical symbiotic counterparts. It is also adapted to prolonged aerial exposure and greater temperature extremes; the animal feeds and its symbionts photosynthesize at temperatures approaching 5°C.” - <span class="citation" data-cites="mccloskey1996">McCloskey, Cove, and Verde (<a href="#ref-mccloskey1996" role="doc-biblioref">1996</a>)</span></p>
<div class="no-row-height column-margin column-container"></div></blockquote>
Expand All @@ -192,7 +193,7 @@ <h1 class="title">The aggregating anemone, Anthopleura elegantissima</h1>
<div class="no-row-height column-margin column-container"></div><blockquote class="blockquote page-columns page-full">
<p>Sea anemones of the genus Anthopleura along northeast Pacific intertidal shores engage in a particularly unique symbiosis with two especially phylogenetically and physiologically different symbionts: the chlorophyte <em>Elliptochloris marina</em> (Letsch et al., 2009) and the dinoflagellate <em>Symbiodinium muscatinei</em> (LaJeunesse and Trench, 2000). - <span class="citation" data-cites="dimond2017">Dimond et al. (<a href="#ref-dimond2017" role="doc-biblioref">2017</a>)</span></p>
<div class="no-row-height column-margin column-container"></div></blockquote>
<p>Most anemones only host one symbiont, or the other. It is rare for mixed assemblages of the two ‘symbiont morphs’ to occur. In the paper by <span class="citation" data-cites="mccloskey1996">McCloskey, Cove, and Verde (<a href="#ref-mccloskey1996" role="doc-biblioref">1996</a>)</span>, they found that among 91 anemones, &gt;95% had a single symbiont type. The chlorophyte <em>Elliptochloris marina</em> is relatively sensitive to light and is limited to cooler, low light environments typical of the low intertidal. The dinoflagellate <em>Symbiodinium muscatinei</em>, on the other hand, tolerates higher light and temperatures and is more comonly found in anemones in the upper intertidal zone. Aposymbiotic (non-symbiont forming) <em>A. elegantissima</em> anemones can be found thriving in very low light conditions like inside rocky crevasses within the intertidal zone. Transplantation of <em>A. elegantissima</em> anemones with chlorophyte <em>E. marina</em> symbionts to warmer, higher-light environments results in shifts in the symbiont to the dinoflagellate <em>S. muscatinei</em> <span class="citation" data-cites="dimond2017">(<a href="#ref-dimond2017" role="doc-biblioref">Dimond et al. 2017</a>)</span>.</p>
<p>Most anemones only host one symbiont, or the other. It is rare for mixed assemblages of the two ‘symbiont morphs’ to occur. In the paper by <span class="citation" data-cites="mccloskey1996">McCloskey, Cove, and Verde (<a href="#ref-mccloskey1996" role="doc-biblioref">1996</a>)</span>, they found that among 91 anemones, &gt;95% had a single symbiont type. The chlorophyte <em>Elliptochloris marina</em> is relatively sensitive to light and is limited to cooler, low light environments typical of the low intertidal. The dinoflagellate <em>Symbiodinium muscatinei</em>, on the other hand, tolerates higher light and temperatures and is more commonly found in anemones in the upper intertidal zone. Aposymbiotic (non-symbiont forming) <em>A. elegantissima</em> anemones can be found thriving in very low light conditions, often inside rocky crevasses within the intertidal zone. Transplantation of <em>A. elegantissima</em> anemones with chlorophyte <em>E. marina</em> symbionts to warmer, higher-light environments results in shifts in the symbiont population from <em>E. marina</em> to <em>S. muscatinei</em> <span class="citation" data-cites="dimond2017">(<a href="#ref-dimond2017" role="doc-biblioref">Dimond et al. 2017</a>)</span>.</p>
<div class="no-row-height column-margin column-container"><div id="ref-mccloskey1996" class="csl-entry" role="doc-biblioentry">
McCloskey, L. R., Timothy G. Cove, and E.Alan Verde. 1996. <span>“Symbiont Expulsion from the Anemone <span>Anthopleura</span> Elegantissima (<span>Brandt</span>) (<span>Cnidaria</span>; <span>Anthozoa</span>).”</span> <em>Journal of Experimental Marine Biology and Ecology</em> 195 (2): 173–86. <a href="https://doi.org/10.1016/0022-0981(95)00079-8">https://doi.org/10.1016/0022-0981(95)00079-8</a>.
</div></div><p>Anemones hosting <em>Symbiodinium muscatinei</em> have a golden-brown color, while those that host <em>Elliptochloris marina</em> are mossy green. Symbiont type can be verified by viewing excised tentacles under light microscopy, and commonly, no mixed-symbiont populations are observed <span class="citation" data-cites="dimond2017">(<a href="#ref-dimond2017" role="doc-biblioref">Dimond et al. 2017</a>)</span>.</p>
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Expand Up @@ -184,7 +184,7 @@ <h2 id="toc-title">Contents <i class="bi bi-bookmark-heart"></i></h2>
<h2 class="anchored" data-anchor-id="conceptual-planning">Conceptual planning</h2>
<section id="questions" class="level3">
<h3 class="anchored" data-anchor-id="questions">Question(s)</h3>
<p>::: callout-note Overarching question Does chronic microplastic-associated leachate pollution reduce marine anthozoan’s climate resilience to marine heatwaves? :::</p>
<p>Does chronic microplastic-associated leachate pollution reduce Cnidarian’s resilience to marine heatwaves?</p>
<ol start="2" type="1">
<li><p><strong><em>Photobiology:</em></strong></p></li>
<li><p><strong><em>Microbiome:</em></strong> Does exposure to microplastic-associated leachate pollution disrupt the beneficial function of the anthozoan microbiome community? Is microbial community function disrupted more under added thermal stress?</p></li>
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