This repository contains data access and computational analysis for the methods developed in our manuscript in Angewandte Chemie.

Data

All the sequencing data have been deposited in the ArrayExpress database at EMBL-EBI under accession number E-MTAB-6456.

Code

• ODN 1 experiment: synthetic oligonucleotide bearing two 5hmUs at defined positions and proximal non-modified T sites. It also contains two 10-mer barcodes to identify unique reads and eliminate potential PCR artefacts.
• ODN 2 experiment: synthetic oligonucleotide bearing two 5hmUs at defined positions and proximal non-modified T sites. 5hmU was incorporated at different levels (%).
• ODN 3 experiment: synthetic oligonucleotide bearing one 5hmU at a defined position, all neighbouring A, C, G, T base combinations introduced at random and 6-mer barcodes to identify unique reads and eliminate potential PCR artefacts.
• Trypanosoma brucei chromosome 2: demonstration of mapping single-base 5hmU sites and chemical-enrichment 5hmU regions in genomic DNA and resulting tables.