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Hi:
In your process : after split the raw fastqs into dna.fasta and rna.fastq, while mapping them with genome.fasta , you use bowtie2 and right after samtools convert to bam.
my question is : why don't u produce sam files and 'samtools view -F 4 ' and awk to make theme simple.txt files?
I think if doing this we can save time and memory, looking forword to your reply!
any help will be grateful!
Haifeng Sun
China NanJing Medical University
20181114
The text was updated successfully, but these errors were encountered:
Hi:
In your process : after split the raw fastqs into dna.fasta and rna.fastq, while mapping them with genome.fasta , you use bowtie2 and right after samtools convert to bam.
my question is : why don't u produce sam files and 'samtools view -F 4 ' and awk to make theme simple.txt files?
I think if doing this we can save time and memory, looking forword to your reply!
any help will be grateful!
Haifeng Sun
China NanJing Medical University
20181114
The text was updated successfully, but these errors were encountered: