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why don‘t bowtie2 produce sam files directly? #2

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sunhaifeng123 opened this issue Nov 14, 2018 · 0 comments
Open

why don‘t bowtie2 produce sam files directly? #2

sunhaifeng123 opened this issue Nov 14, 2018 · 0 comments

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@sunhaifeng123
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Hi:
In your process : after split the raw fastqs into dna.fasta and rna.fastq, while mapping them with genome.fasta , you use bowtie2 and right after samtools convert to bam.
my question is : why don't u produce sam files and 'samtools view -F 4 ' and awk to make theme simple.txt files?
I think if doing this we can save time and memory, looking forword to your reply!
any help will be grateful!
Haifeng Sun
China NanJing Medical University
20181114

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