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Blast Alignments.txt
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Blast Alignments.txt
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Primer pair 1
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer CGTACGTGGCTTTGGAGACT Plus 20 81 100 60.04 55.00 6.00 3.00
Reverse primer ACCATGAGGTGCAGTTCGAG Minus 20 246 227 60.04 55.00 4.00 2.00
Product length 166
Primer pair 2
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer TCGTACGTGGCTTTGGAGAC Plus 20 80 99 60.04 55.00 6.00 1.00
Reverse primer AGATCGGCGCCGTAACTATG Minus 20 419 400 60.04 55.00 8.00 2.00
Product length 340
Primer pair 3
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer ACCGCAAGGTTCTTCTTCGT Plus 20 353 372 59.89 50.00 3.00 0.00
Reverse primer CAAGCTCGTCGCCTAAGTCA Minus 20 448 429 60.11 55.00 4.00 1.00
Product length 96
Primer pair 4
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer CGGTCGTAGTGGTGAGACAC Plus 20 291 310 60.11 60.00 3.00 3.00
Reverse primer ACGAAGAAGAACCTTGCGGT Minus 20 372 353 59.89 50.00 3.00 2.00
Product length 82
Primer pair 5
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer GAGCCTTGTCCCTGGTTTCA Plus 20 6 25 59.89 55.00 3.00 1.00
Reverse primer AAGAACCTTGCGGTAAGCCA Minus 20 366 347 59.89 50.00 5.00 1.00
Product length 361
Primer pair 6
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer TACGGCGCCGATCTAAAGTC Plus 20 406 425 59.97 55.00 8.00 2.00
Reverse primer CACGGGTAACACCACTGCTA Minus 20 514 495 59.68 55.00 3.00 2.00
Product length 109
Primer pair 7
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer TGGCTTACCGCAAGGTTCTT Plus 20 347 366 59.89 50.00 5.00 3.00
Reverse primer ACGCATGAGTTCACGGGTAA Minus 20 525 506 59.68 50.00 6.00 2.00
Product length 179
Primer pair 8
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer CTCGTACGTGGCTTTGGAGA Plus 20 79 98 59.76 55.00 6.00 0.00
Reverse primer TGCCTTCGAGTTCTGCTACC Minus 20 283 264 59.75 55.00 4.00 2.00
Product length 205
Primer pair 9
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer AGCTGGTGGCCATAGTTACG Plus 20 390 409 59.82 55.00 6.00 2.00
Reverse primer CCTCCGTTAAGCTCACGCAT Minus 20 539 520 60.46 55.00 4.00 2.00
Product length 150
Primer pair 10
Sequence (5'->3') Template strand Length Start Stop Tm GC% Self complementarity Self 3' complementarity
Forward primer ATGCTCGAACTGCACCTCAT Plus 20 224 243 59.75 50.00 4.00 2.00
Reverse primer GGACACCAAGTGTCTCACCA Minus 20 319 300 59.53 55.00 7.00 0.00
Product length 96