Skip to content

tim-inzitari/BioInformatics-PCR-Project

Folders and files

NameName
Last commit message
Last commit date

Latest commit

 

History

25 Commits
 
 
 
 
 
 
 
 

Repository files navigation

BioInformatics-PCR-Project

for class

Notes

Since BLAST does not give primers that align perfectly, and we are only going to allow perfect allignments, we must pick our own primers.

Currently these primers are selected by first randomly selecting a sub-region of our gene that is between 150-250 nucleotides long, and then using the first 20 and last 20 nucleotides as forward and reverse primers (the reverse primer being the compliment of course).

The simulation is done in two "buckets" which account for the strands that are duplicated in the forward and reverse way. This simplification makes the solution much easier to follow.

Once the simulation is over it plots the lengths of all the strands on a histogram.

Releases

No releases published

Packages

No packages published

Languages