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Update README.md
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xiaoli-dong authored Nov 2, 2023
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Expand Up @@ -42,7 +42,7 @@ By default, the pipeline supports both short and long reads:
- Nanopore long read quality and length filter ([chopper](https://github.com/wdecoster/chopper))
- Nanopore long read statistics ([seqkit stats](https://bioinf.shenwei.me/seqkit/usage/#stats))
- Assembly
- Short read assembly with user choice of the assemblers ([Spades](https://github.com/ablab/spades) | [Skesa](https://github.com/ncbi/SKESA) | [Unicycler](https://github.com/rrwick/Unicycler) | [megahit](https://github.com/voutcn/megahit))
- Short read assembly with user choice of the assemblers ([Spades](https://github.com/ablab/spades) | [Skesa](https://github.com/ncbi/SKESA) | [Unicycler](https://github.com/rrwick/Unicycler) | [megahit](https://github.com/voutcn/megahit) | [shovill](https://github.com/tseemann/shovill)
- Long read assembly is following the steps below:
- Nanopore long read de novo assembly ([`Flye`](https://github.com/fenderglass/Flye))
- Circular Flye contigs are rotated to start in the center of the contig ([in-house perl script](https://github.com/xiaoli-dong/pathogenseq/blob/main/bin/reset_start_position_for_circular_genome.pl))
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