feat: heterogeneous feature generation, img_utils

src/deep_neurographs/geometry.py

@@ -291,7 +291,7 @@ def fill_path(img, path, val=-1):
     """
     for xyz in path:
         x, y, z = tuple(np.floor(xyz).astype(int))
-        img[x - 1 : x + 2, y - 1 : y + 2, z - 1 : z + 2] = val
+        img[x - 1: x + 2, y - 1: y + 2, z - 1: z + 2] = val
     return img
 
 
@@ -425,9 +425,9 @@ def align(neurograph, img, branch_1, branch_2, depth):
     best_d2 = None
     best_score = 0
     for d1 in range(min(depth, len(branch_1) - 1)):
-        xyz_1 = neurograph.to_img(branch_1[d1], shift=True)
+        xyz_1 = neurograph.to_voxels(branch_1[d1], shift=True)
         for d2 in range(min(depth, len(branch_2) - 1)):
-            xyz_2 = neurograph.to_img(branch_2[d2], shift=True)
+            xyz_2 = neurograph.to_voxels(branch_2[d2], shift=True)
             line = make_line(xyz_1, xyz_2, 10)
             score = np.mean(get_profile(img, line, window=[3, 3, 3]))
             if score > best_score:

src/deep_neurographs/img_utils.py

@@ -0,0 +1,202 @@
+"""
+Created on Sat May 9 11:00:00 2024
+
+@author: Anna Grim
+@email: anna.grim@alleninstitute.org
+
+Helper routines for working with images.
+
+"""
+
+from concurrent.futures import ThreadPoolExecutor
+from copy import deepcopy
+
+import numpy as np
+import tensorstore as ts
+import zarr
+from skimage.color import label2rgb
+
+SUPPORTED_DRIVERS = ["neuroglancer_precomputed", "n5", "zarr"]
+
+
+# --- io utils ---
+def open_tensorstore(path, driver):
+    """
+    Uploads segmentation mask stored as a directory of shard files.
+
+    Parameters
+    ----------
+    path : str
+        Path to directory containing shard files.
+    driver : str
+        Storage driver needed to read data at "path".
+
+    Returns
+    -------
+    sparse_volume : dict
+        Sparse image volume.
+
+    """
+    assert driver in SUPPORTED_DRIVERS, "Error! Driver is not supported!"
+    arr = ts.open(
+        {
+            "driver": driver,
+            "kvstore": {
+                "driver": "gcs",
+                "bucket": "allen-nd-goog",
+                "path": path,
+            },
+            "context": {
+                "cache_pool": {"total_bytes_limit": 1000000000},
+                "cache_pool#remote": {"total_bytes_limit": 1000000000},
+                "data_copy_concurrency": {"limit": 8},
+            },
+            "recheck_cached_data": "open",
+        }
+    ).result()
+    if driver == "neuroglancer_precomputed":
+        return arr[ts.d["channel"][0]]
+    elif driver == "zarr":
+        arr = arr[0, 0, :, :, :]
+        arr = arr[ts.d[0].transpose[2]]
+        arr = arr[ts.d[0].transpose[1]]
+    return arr
+
+
+def open_zarr(path):
+    """
+    Opens zarr file at "path".
+
+    Parameters
+    ----------
+    path : str
+        Path to zarr file to be opened.
+
+    Returns
+    -------
+    np.ndarray
+        Contents of zarr file at "path".
+
+    """
+    n5store = zarr.N5FSStore(path, "r")
+    if "653980" in path:
+        return zarr.open(n5store).ch488.s0
+    elif "653158" in path:
+        return zarr.open(n5store).s0
+
+
+def read_tensorstore(arr, xyz, shape, from_center=True):
+    """
+    Reads a chunk of data from the specified tensorstore array, given the
+    coordinates and shape of the chunk.
+
+    Parameters
+    ----------
+    arr : tensorstore.ndarray Array
+        Array from which data is to be read.
+    xyz : tuple
+        xyz coordinates of chunk to be read from the tensorstore array.
+    shape : tuple
+        Shape (dimensions) of the chunk to be read.
+    from_center : bool, optional
+        Indication of whether the provided coordinates represent the center of
+        the chunk or the starting point. If True, coordinates are the center;
+        if False, coordinates are the starting point of the chunk. The default
+        is True.
+
+    Returns
+    -------
+    numpy.ndarray
+        Chunk of data read from the tensorstore array.
+
+    """
+    chunk = read_chunk(arr, xyz, shape, from_center=from_center)
+    return chunk.read().result()
+
+
+def read_tensorstore_with_bbox(img, bbox):
+    start = bbox["min"]
+    end = bbox["max"]
+    return (
+        img[start[0]: end[0], start[1]: end[1], start[2]: end[2]]
+        .read()
+        .result()
+    )
+
+
+def read_chunk(arr, xyz, shape, from_center=True):
+    start, end = get_start_end(xyz, shape, from_center=from_center)
+    return deepcopy(
+        arr[start[0]: end[0], start[1]: end[1], start[2]: end[2]]
+    )
+
+
+def get_start_end(xyz, shape, from_center=True):
+    if from_center:
+        start = [xyz[i] - shape[i] // 2 for i in range(3)]
+        end = [xyz[i] + shape[i] // 2 for i in range(3)]
+    else:
+        start = xyz
+        end = [xyz[i] + shape[i] for i in range(3)]
+    return start, end
+
+
+def read_superchunks(img_path, labels_path, xyz, shape, from_center=True):
+    with ThreadPoolExecutor() as executor:
+        img_job = executor.submit(
+            get_superchunk,
+            img_path,
+            "n5" if ".n5" in img_path else "zarr",
+            xyz,
+            shape,
+            from_center=from_center,
+        )
+        labels_job = executor.submit(
+            get_superchunk,
+            labels_path,
+            "neuroglancer_precomputed",
+            xyz,
+            shape,
+            from_center=from_center,
+        )
+    img = img_job.result().astype(np.int16)
+    labels = labels_job.result().astype(np.int64)
+    assert img.shape == labels.shape, "img.shape != labels.shape"
+    return img, labels
+
+
+def get_superchunk(path, driver, xyz, shape, from_center=True):
+    arr = open_tensorstore(path, driver)
+    return read_tensorstore(arr, xyz, shape, from_center=from_center)
+
+
+# -- Image Operations --
+def normalize_img(img):
+    img -= np.min(img)
+    return img / np.max(img)
+
+
+def get_mip(img, axis=0):
+    """
+    Compute the maximum intensity projection (MIP) of "img" along "axis".
+
+    Parameters
+    ----------
+    img : numpy.ndarray
+        Image to compute MIP of.
+    axis : int, optional
+        Projection axis. The default is 0.
+
+    Returns
+    -------
+    numpy.ndarray
+        MIP of "img".
+
+    """
+    return np.max(img, axis=axis)
+
+
+def get_labels_mip(img, axis=0):
+    mip = np.max(img, axis=axis)
+    mip = label2rgb(mip)
+    return (255 * mip).astype(np.uint8)