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30 changes: 16 additions & 14 deletions CITATION.cff
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Expand Up @@ -88,35 +88,37 @@ authors:
Unit “Infection and Public Health”, Paterna, Spain
identifiers:
- type: doi
value: 10.1101/2023.10.24.561010
description: Preprint
value: 10.1093/ve/veae018
description: Journal article
- type: doi
value: 10.20350/digitalCSIC/15648
description: >-
Case study data: SARS-CoV-2 mapped reads and consensus
genomes of an intra-patient serially sampled infection
repository-code: 'https://github.com/PathoGenOmics-Lab/VIPERA'
url: 'https://doi.org/10.1101/2023.10.24.561010'
url: 'https://doi.org/10.1093/ve/veae018'
abstract: >-
Viral mutations within patients nurture the adaptive
potential of SARS-CoV-2 during chronic infections, which
are a potential source of variants of concern. However,
there is no integrated framework for the evolutionary
analysis of intrapatient SARS-CoV-2 serial samples. Herein
we describe VIPERA (Viral Intra-Patient Evolution
Reporting and Analysis), a new software that integrates
the evaluation of the intra-patient ancestry of SARS-CoV-2
potential of severe acute respiratory syndrome coronavirus
2 (SARS-CoV-2) during chronic infections, which are a
potential source of variants of concern. However, there is
no integrated framework for the evolutionary analysis of
intra-patient SARS-CoV-2 serial samples. Herein, we
describe Viral Intra-Patient Evolution Reporting and
Analysis (VIPERA), a new software that integrates the
evaluation of the intra-patient ancestry of SARS-CoV-2
sequences with the analysis of evolutionary trajectories
of serial sequences from the same viral infection. We have
validated it using positive and negative control datasets
and have successfully applied it to a new case, thus
enabling an easy and automatic analysis of intra-patient
SARS-CoV-2 sequences.
and have successfully applied it to a new case, which
revealed population dynamics and evidence of adaptive
evolution. VIPERA is available under a free software
license at https://github.com/PathoGenOmics-Lab/VIPERA.
keywords:
- SARS-CoV-2
- Intra-host viral evolution
- Chronic infection
- Bioinformatics
- Snakemake
license: GPL-3.0
version: 1.0.0
version: 1.2.0
25 changes: 15 additions & 10 deletions README.md
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Expand Up @@ -5,7 +5,7 @@
</p>

[![PGO badge](https://img.shields.io/badge/PathoGenOmics-Lab-yellow.svg)](https://pathogenomics.github.io/)
[![DOI:10.1101/2023.10.24.561010](https://img.shields.io/badge/DOI-10.1101/2023.10.24.561010-blue.svg)](https://doi.org/10.1101/2023.10.24.561010)
[![DOI](https://img.shields.io/badge/Virus_Evolution-10.1093/ve/veae018-387088.svg)](https://doi.org/10.1093/ve/veae018)
[![Release](https://img.shields.io/github/v/release/PathoGenOmics-Lab/VIPERA)](https://github.com/PathoGenOmics-Lab/VIPERA/releases)
[![Snakemake](https://img.shields.io/badge/Snakemake-≥7.19-brightgreen.svg?style=flat)](https://snakemake.readthedocs.io)
![Install workflow](https://github.com/PathoGenOmics-Lab/VIPERA/actions/workflows/install.yml/badge.svg)
Expand All @@ -24,7 +24,7 @@ configuring [the inputs and outputs](config/README.md#inputs-and-outputs) and
snakemake --use-conda -c4 # runs VIPERA on 4 cores
```

We provide a simple script that downloads the [data](https://doi.org/10.20350/digitalCSIC/15648) from [our study](https://doi.org/10.1101/2023.10.24.561010)
We provide a simple script that downloads the [data](https://doi.org/10.20350/digitalCSIC/15648) from [our study](https://doi.org/10.1093/ve/veae018)
and performs the analysis in a single step:

```shell
Expand All @@ -49,18 +49,23 @@ Please refer to the [full workflow documentation](config/README.md) for detailed

## Citation

Álvarez-Herrera M. & Sevilla, J., Ruiz-Rodriguez, P., Vergara, A., Vila, J., Cano-Jiménez, P., González-Candelas, F., Comas, I., & Coscolla, M. (2023). VIPERA: Viral Intra-Patient Evolution Reporting and Analysis. bioRxiv. https://doi.org/10.1101/2023.10.24.561010
> Álvarez-Herrera, M. & Sevilla, J., Ruiz-Rodriguez, P., Vergara, A., Vila, J., Cano-Jiménez, P., González-Candelas, F., Comas, I., & Coscollá, M. (2024). VIPERA: Viral Intra-Patient Evolution Reporting and Analysis. Virus Evolution, 10(1), veae018. https://doi.org/10.1093/ve/veae018
```bibtex
@misc{AHS_VIPERA_2023,
@article{alvarez-herrera_vipera_2024,
title = {{VIPERA}: {Viral} {Intra}-{Patient} {Evolution} {Reporting} and {Analysis}},
volume = {10},
issn = {2057-1577},
shorttitle = {{VIPERA}},
author = {Álvarez-Herrera$^*$, Miguel and Sevilla$^*$, Jordi and Ruiz-Rodriguez, Paula and Vergara, Andrea and Vila, Jordi and Cano-Jiménez, Pablo and González-Candelas, Fernando and Comas, Iñaki and Coscolla, Mireia},
url = {https://www.biorxiv.org/content/10.1101/2023.10.24.561010},
doi = {10.1101/2023.10.24.561010},
language = {en},
urldate = {2023-10-25},
publisher = {bioRxiv},
url = {https://doi.org/10.1093/ve/veae018},
doi = {10.1093/ve/veae018},
abstract = {Viral mutations within patients nurture the adaptive potential of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) during chronic infections, which are a potential source of variants of concern. However, there is no integrated framework for the evolutionary analysis of intra-patient SARS-CoV-2 serial samples. Herein, we describe Viral Intra-Patient Evolution Reporting and Analysis (VIPERA), a new software that integrates the evaluation of the intra-patient ancestry of SARS-CoV-2 sequences with the analysis of evolutionary trajectories of serial sequences from the same viral infection. We have validated it using positive and negative control datasets and have successfully applied it to a new case, which revealed population dynamics and evidence of adaptive evolution. VIPERA is available under a free software license at https://github.com/PathoGenOmics-Lab/VIPERA.},
number = {1},
journal = {Virus Evolution},
author = {Álvarez-Herrera$^*$, Miguel and Sevilla$^*$, Jordi and Ruiz-Rodriguez, Paula and Vergara, Andrea and Vila, Jordi and Cano-Jiménez, Pablo and González-Candelas, Fernando and Comas, Iñaki and Coscollá, Mireia},
month = jan,
year = {2024},
pages = {veae018},
note = {$^*$ indicates equal contribution}
}
```

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