-
Notifications
You must be signed in to change notification settings - Fork 0
2nd Meeting Notes, Nov 30th
Xueqing edited this page Jan 6, 2018
·
1 revision
- The transcripts have been preliminarily mapped to Ensembl reference genes (by Muhammad and Dimitri).
- The gene lengths (exonic) are added to calculate TPM. If TPM < 1, the genes are not differentially expressed (ref to be attached by Muhammad).
- The first filter should be based on the protein-coding genes in HPA db. Then do differential expression analysis.
- Sequencing platform: Illumina HiSeq
To-Do List (by the end of this week):
Combine metadata and raw counts.
Look up whether to sum up or take median/mean for the samples that were sequenced multiple times (deeper coverage).
The transcripts have been preliminarily mapped to Ensembl reference genes.
Upload reference regarding TPM value cut-off.