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gc bias metrics

jprofijt edited this page Feb 17, 2020 · 1 revision

GC bias metrics

detailed metrics about reads that fall within windows of a certain GC bin on the reference genome.

Attribute Description
ACCUMILATION_LEVEL ?
READS_USED This option is used to mark including or excluding duplicates.
GC The G+C content of the reference sequence represented by this bin
WINDOWS The number of windows on the reference genome that have this G+C content
READ_STARTS The number of reads whose start position is at the start of a window of this GC
MEAN_BASE_QUALITY The mean quality of all bases of all reads that are assigned to windows of this GC
NORMALIZED_COVERAGE the ration of "coverage" in this GC bin vs. the mean coverage of all GC bins. 1 represents mean coverage, < 1 is lower than mean coverage, > 1 means more coverage than the mean
SAMPLE The sample to which these metrics apply. If null, it means they apply to all reads in the file.
LIBRARY The library to which these metrics apply. If null, it means that the metrics were accumulated at the sample level.
READ_GROUP The read group to which these metrics apply. If null, it means that the metrics were accumulated at the library or sample level.
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