Flow Diagram

Flow chart

CORASON will do for you the following steps:

1. Look for clusters with an ortholog of your query protein and create a PrincipalHits quicktree (1_Context_text.pl)
2. Extract from those clusters the ones that share at least other ortholog with your query cluster(ReadingInputs.pl)
3. Look for the genomic core on clusters, each cluster is a MINI genome. (perl 2_OrthoGroups.pl)
   If exists a genomic core on clusters, then:

• Align, shave and concatenate the core cluster proteins on a fasta file ready to MrBayes.
• Create a quick tree with the concatenated aminoacid matrix of genomic core on clusters.

4. Draw an SVG file of the hit-clusters sorted according either to PrincipalHits or to multilocus core genomic tree (3_Draw.pl)
5. Write an functional report of core enzymes if core was found.