edge-detection image-segmentation motion-analysis cell-division jacobian-matrix mitotic-cells-segmentation difference-of-gaussian protein-translocation local-gradient texture-filter fluorescent-speckle-microscopy ratio-of-eigenvalues wrapped-around-colormap orientation-sensetive-filter scale-space-speckles local-flow-direction hierarchical-feature-detection local-motion-orientation biological-function-analysis mitotic-spindle