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DISCLAIMER: this resource should be viewed as an add-on to standard protocol for rodent stereotaxic surgeries. Standard protocols have been published earlier (Cetin et al., Nat. Protocol. 2007, and others).
IMPORTANT: Those procedures must be approved by Institutional Animal Care and Use Committee (IACUC) and Institutional Biosafety Committee (IBC) and performed under aseptic conditions described in those approved procedures
This is an overview of a protocol for mouse stereotaxic surgeries. The main features of this protocol are:
- Stereotaxic robot (external product website)
- Low-flow anesthesia unit (external product website)
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ctl+clickto open links in new tab (blue/underline blue text) -
Settings/playback speedchange the playback speed for videos. Videos are recorded in realtime to provide an estimate of the time required per operation.
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Stereotax
- syringe
- drill
- ferrule
- bregma
- automatic bregma finder
- manual bregma finder
- Anesthesia
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Stereotax
- manual calibration for (syringe, ferrule and drill)
- automatic calibration for (syringe and drill)
- automatic calibration for (ferrule and drill)
Shortcuts (list)
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Syringe:setting up and loading the syringe (video) -
Drill:changing the end mill (video) -
Cannula & Ferrule:setting them up (video) -
Calibration:stereotaxic tools calibration on a reference point (video) -
Calibration:drill and syringe auto-calibration (no cannula should be present) (video) -
Bregma Finder:use the camera so that the bregma finder can easily access bregma (not yet available) -
Anesthesia Unit:setting up the anesthesia unit (video) -
Surgery:mounting the animal in the stereotax (video) -
Surgery:example surgery for virus injection and cannula implantation (video) -
Surgery:example surgery for craniotomy (not yet available)