CraniotomyOverview
Use a 0.2mm drill bit (cat#13908,Harvey Tool - machineshop to fit the drill)
* For S1 center AP=-2.0, ML=-3.5 Circle = 1.50 mm
* For Thal AP=-1.5, ML=-1.0 Circle = 1.00 mm
* For M1 AP=-1.0, ML=-1.0 Circle = 1.00 mm
* set bregma and lambda (could set only bregma if needed)
* set the skull thickness to 2.00 mm (necessary to reach very lateral area S1)
a. check Auto-speed
b. keyboard short cuts F7 = Auto-speed
c. keyboard short cuts F8 = 1 mm/min <=> 0.016 mm/s
* Saftey above skull = 0.4 mm
* Drill approach = 0.2 mm
* Approaching speed = 1 mm/s
* Drilling speed = 0.05 mm/s (# speed could be doubled)
* Extracting drill = 1 mm/s
* Drill mode craniotomy shape
* Check box Auto-Stop on High setings
* Press F7 to activate Auto-Speed feature
* Start Auto Scan
* When the drill start dirlling the bone:
* press ESC
* press F8 to activate 0.016 mm/s low drilling speed
* When the drill stop to switch to the next target
* press ESC
* press F7 to activate Auto-Speed feature
* press Auto Scan
* (repeat those steps for all the seed points)
* Go to Correct tilt and scaling and set skull thickness back to 0.4 mm in mouse skull thickness
* between (skull thickness [ref](https://doi.org/10.1117/1.JBO.22.1.010503))
* Auto cut off set of 200 um.
* Press F7 to activate the speed
* Deactivate the Auto-Stop feature
* Soak the skull surface with Ringers
* Edge cut Horizontally
* Note if edge cut is set to 200 um the drill will do to itteration of circles of 100 um each
* (this can be modified in the cut options)
* 0’ - start surgery
* 3’ - measure bregma
* 5’ - start seed acquisition
* 11’ - end seed acquisition
* 12’ - start horizontal cut
* 16 - end horizontal cut
